IP Library › Patent Application 16795203
Patent Application
App. No. 16/795,203

METHODS OF IDENTIFYING MULTIPLE EPITOPES IN CELLS

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Patent No.
US None
App. No.
16/795,203
Abstract

The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.

Claims (24)

1 . An automated system comprising:

a liquid handling platform comprising a modular microplate assembly including a plurality of microplate positions and a pipette head movable to the plurality of microplate positions;

at least one microplate storage unit;

a robotic arm movable between at least the plurality of microplate positions and the at least one microplate storage unit; and

a controller communicatively coupled to at least the liquid handling platform and the robotic arm.

2 . The automated system of claim 1 , wherein the at least one microplate storage unit comprises a stacking tower.

3 . The automated system of claim 1 , further comprising a second microplate storage unit, and wherein the robotic arm is movable to the second microplate storage unit.

4 . The automated system of claim 1 , wherein the liquid handling platform further comprises a gripper.

5 . The automated system of claim 1 , wherein the pipette head comprises an interchangeable pipette head.

6 . The automated system of claim 5 , wherein the interchangeable pipette head comprises 96 pipette channels.

7 . The automated system of claim 5 , wherein the interchangeable pipette head comprises 384 pipette channels.

8 . The automated system of claim 1 , wherein at least one of the plurality of microplate positions comprises a heating element.

9 . The automated system of claim 1 , wherein at least one of the plurality of microplate positions comprises a cooling element.

10 . The automated system of claim 1 , further comprising a microplate sealing device.

11 . The automated system of claim 10 , further comprising a microplate sealant removal device.

12 . The automated system of claim 1 , further comprising at least one of a microplate shaker and a centrifuge.

13 . The automated system of claim 1 , further comprising at least one of an incubator and a thermocycler.

14 . The automated system of claim 1 , further comprising a sequencing device.

15 . The automated system of claim 1 , further comprising a magnetic separation device.

16 . The automated system of claim 1 , further comprising a reagent storage vessel.

17 . The automated system of claim 1 , further comprising at least one of a microplate reader and a fluorescent detector.

18 . The automated system of claim 1 , wherein the controller comprises one or more central processing units and one or more memories communicatively coupled thereto, wherein the one or more memories include instructions for performing a plurality of liquid and/or cell handling procedures.

19 . The automated system of claim 19 , wherein the plurality of liquid and/or cell handling procedures are adapted for performing split-pool synthesis.

20 . The automated system of claim 1 , wherein the automated system is configured to perform one or more steps of split-pool synthesis.