IP Library › Granted Patent US 10,655,114
Granted Patent B1
US 10,655,114 · App. 16/798,309 · Granted May 19, 2020

Engineered enzymes

Inventors: Benjamin Mijts (Boulder, CO); Juhan Kim (Boulder, CO); Aamir Mir (Boulder, CO); Kyle Seamon (Boulder, CO); Andrew Garst (Boulder, CO)
Assignee: Inscripta, Inc.
C12N9/22
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Quick Facts
Patent No.
US 10,655,114
App. No.
16/798,309
Granted
May 19, 2020
Kind
B1
Abstract

The present disclosure provides engineered RNA-guided enzymes for editing live cells.

Claims (20)

1. An engineered nucleic acid-guided nuclease having editing activity in yeast different than the nucleic acid-guided nuclease having the sequence of SEQ ID No. 1, wherein the engineered nucleic acid-guided nuclease has a sequence comprising any of SEQ ID Nos. 69-78 or 79-86.

2. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 69.

3. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 70.

4. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 71.

5. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 72.

6. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 73.

7. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 74.

8. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 75.

9. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 76.

10. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 77.

11. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 78.

12. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 79.

13. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 80.

14. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 81.

15. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 82.

16. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 83.

17. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 84.

18. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 85.

19. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 86.

20. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 .

Assignments (3)
MERGER AND CHANGE OF NAME Recorded Sep 15, 2025
From: INSCRIPTA, INC.,; MANUS INSCRIPTA, INC.
To: MANUS INSCRIPTA, INC.
Reel/Frame 072252/0203 →
SECURITY INTEREST Recorded Sep 8, 2025
From: MANUS BIO INC.; STO.PERU I LLC; STO.PERU II LLC; MANUS INTERMEDIATE INC.; MANUS INSCRIPTA, INC.
To: SYMBIOTIC CAPITAL AGENCY LLC, AS ADMINISTRATIVE AND COLLATERAL AGENT
Reel/Frame 072836/0255 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 22, 2020
From: MIJTS, BENJAMIN; KIM, JUHAN; MIR, AAMIR; SEAMON, KYLE; GARST, ANDREW
To: INSCRIPTA, INC.
Reel/Frame 051895/0269 →
Continuity (2)
Continuation 16658948 · Oct 21, 2019
Provisional Application 62748668 · Oct 22, 2018