IP Library › Granted Patent US 10,640,754
Granted Patent B1
US 10,640,754 · App. 16/798,315 · Granted May 5, 2020

Engineered enzymes

Inventors: Benjamin Mijts (Boulder, CO); Juhan Kim (Boulder, CO); Aamir Mir (Boulder, CO); Kyle Seamon (Boulder, CO); Andrew Garst (Boulder, CO)
Assignee: Inscripta, Inc.
C12N9/22
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Quick Facts
Patent No.
US 10,640,754
App. No.
16/798,315
Granted
May 5, 2020
Kind
B1
Abstract

The present disclosure provides engineered RNA-guided enzymes for editing live cells.

Claims (14)

1. An engineered nucleic acid-guided nuclease with an altered fidelity relative to the nucleic acid-guided nuclease having the sequence of SEQ ID No. 1, wherein the engineered nucleic acid-guided nuclease has a sequence comprising any of SEQ ID Nos. 4-7.

2. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 4.

3. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 5.

4. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 6.

5. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 7.

6. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 .

7. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 2.

8. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 3.

9. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 11.

10. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 12.

11. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 13.

12. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 14.

13. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 67.

14. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 68.

Assignments (3)
MERGER AND CHANGE OF NAME Recorded Sep 15, 2025
From: INSCRIPTA, INC.,; MANUS INSCRIPTA, INC.
To: MANUS INSCRIPTA, INC.
Reel/Frame 072252/0203 →
SECURITY INTEREST Recorded Sep 8, 2025
From: MANUS BIO INC.; STO.PERU I LLC; STO.PERU II LLC; MANUS INTERMEDIATE INC.; MANUS INSCRIPTA, INC.
To: SYMBIOTIC CAPITAL AGENCY LLC, AS ADMINISTRATIVE AND COLLATERAL AGENT
Reel/Frame 072836/0255 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 22, 2020
From: MIJTS, BENJAMIN; KIM, JUHAN; MIR, AAMIR; SEAMON, KYLE; GARST, ANDREW
To: INSCRIPTA, INC.
Reel/Frame 051895/0273 →
Continuity (2)
Continuation 16658948 · Oct 21, 2019
Provisional Application 62748668 · Oct 22, 2018