Catalysis of diketopiperazine synthesis
Provided is a method for the synthesis of N-protected 3,6-bis-[4-aminobutyl]-2,5-diketopiperazine including the step of heating a solution of ε-amino protected lysine in the presence of a catalyst selected from the group consisting of sulfuric acid, phosphoric acid, and phosphorus pentoxide.
1. A method for the synthesis of N-protected 3,6-bis[4-aminoalkyl]-2,5-diketopiperazine comprising:
a. adding an amino acid having a free α-amine group and a protected amine group to a solvent;
b. a step for catalyzing dehydrative cyclocondensation of the amino acid having a free α-amine group and a protected amine group; and
c. heating the amino acid having a free α-amine group and a protected amine group to a target temperature range of between 160° C. and 170° C.,
wherein said step for catalyzing comprises heating said solvent containing said amino acid to said target temperature range is achieved in about 4-6 hours.
2. The method of claim 1 , wherein said step for catalyzing comprises heating said solvent containing said amino acid to a target temperature and the target temperature is maintained for up to 6 hours.
3. The method of claim 1 , wherein said step for catalyzing comprises heating said solvent containing said amino acid to a target temperature and wherein said synthesis is substantially complete within about 8 hours of reaching said target temperature.
4. The method of claim 1 , wherein said step for catalyzing dehydrative cyclocondensation of said amino acid comprises addition of a catalyst to the solution, wherein said catalyst comprises at least one of sulfuric acid, phosphoric acid, or phosphorus pentoxide.