ENGINEERED CRY6A INSECTICIDAL PROTEINS
Engineered and modified Cry6Aa insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
1 . A modified Cry6Aa insecticidal protein comprising modifications chosen from the group consisting of a modified proteolysis-susceptible region, increased affinity of the carboxy terminal peptide (CTP) for the core protein, and addition of sub-cellular transit peptides.
2 . A modified Cry6Aa insecticidal protein of claim 1 chosen from the group consisting of SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:64, SEQ ID NO:66, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, SEQ ID NO:76, SEQ ID NO:78, SEQ ID NO:80, SEQ ID NO:82, SEQ ID NO:84, SEQ ID NO:86, SEQ ID NO:88, SEQ ID NO:90, SEQ ID NO:92, SEQ ID NO:94, SEQ ID NO:96, SEQ ID NO:98, SEQ ID NO:100, SEQ ID NO:102, SEQ ID NO:104, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:110, SEQ ID NO:112, SEQ ID NO:114, SEQ ID NO:116, SEQ ID NO:118, SEQ ID NO:120, SEQ ID NO:132, SEQ ID NO:136, SEQ ID NO:138, SEQ ID NO:140, and SEQ ID NO:144.
3 . A modified Cry6Aa insecticidal protein of claim 1 chosen from the group consisting of SEQ ID NO:110, SEQ ID NO:112, SEQ ID NO:114, SEQ ID NO:116, SEQ ID NO:118, SEQ ID NO:120, SEQ ID NO:128, SEQ ID NO:132, SEQ ID NO:140, and SEQ ID NO:144.
4 . A modified Cry6Aa insecticidal protein of claim 1 chosen from the group consisting of SEQ ID NO:116, SEQ ID NO:118, SEQ ID NO:120, SEQ ID NO:140, SEQ ID NO:144.
5 . A modified Cry6Aa insecticidal protein of claim 1 that is SEQ ID NO:116.
6 . A nucleic acid sequence encoding a modified Cry6Aa insecticidal protein of claim 1 .
7 . A nucleic acid sequence encoding a modified Cry6Aa insecticidal protein of claim 2 .
8 . A nucleic acid sequence encoding a modified Cry6Aa insecticidal protein of claim 3 .
9 . A nucleic acid sequence encoding a modified Cry6Aa insecticidal protein of claim 4 .
10 . A nucleic acid sequence encoding a modified Cry6Aa insecticidal protein of claim 5 .
11 . A DNA construct comprising a nucleic acid sequence of claim 6 operably linked to a promoter that is capable of driving expression in a plant.
12 . A transgenic plant or plant part that comprises a nucleic acid sequence of claim 6 .
13 . A transgenic plant or plant part that comprises a nucleic acid sequence of claim 8 .
14 . A method of controlling insect pest damage to plants which comprises delivering an insecticidally effective amount of an insecticidal protein of claim 1 such that the insect pest ingests said insecticidal protein.
15 . The method of claim 14 wherein the insect pest damage is cause by insects of the order Coleoptera.
16 . The method of claim 15 wherein the insect pest damage is caused by western corn rootworms, Diabrotica virgifera virgifera LeConte.
17 . A method of controlling insect pest damage to plants which comprises preparing a transgenic plant of claim 12 and presenting said plant to an insect pest population.
18 . A method of controlling insect pest damage to plants which comprises preparing a transgenic plant of claim 13 and presenting said plant to an insect pest population.
19 . The method of claim 18 wherein the insect pest damage is cause by insects of the order Coleoptera.
20 . The method of claim 18 wherein the insect pest damage is caused by western corn rootworms, Diabrotica virgifera virgifera LeConte.