Homogenizer and method of grinding large sample quantities
A method of grinding large sample quantities using a bead beater homogenizer includes steps of loading a sample material into a vial having a size of 500 ml or greater having a chamber enclosed by two end walls and a cylindrical sidewall defining a central vial axis, loading a plurality of balls into the vial with the sample material, securing the vial to a movable platform of the homogenizer, and oscillating the platform in a back-and-forth motion isolated to a movement axis, thereby causing the balls to move in a circular motion along the cylindrical sidewall, wherein the central vial axis is perpendicular to the movement axis. A system of a cylindrical vial and a bead beater homogenizer is also provided.
1. A method of grinding large sample quantities using a bead beater homogenizer, the method comprising steps of:
loading a sample material into a vial having a size of 500 ml or greater having a chamber enclosed by two end walls and a cylindrical sidewall defining a central vial axis;
loading a plurality of balls into the vial with the sample material;
securing the vial to a movable platform of the homogenizer; and
oscillating the platform in a back-and-forth motion isolated to a movement axis, thereby causing the balls to move in a circular motion along the cylindrical sidewall, wherein the central vial axis is perpendicular to the movement axis.
2. The method of claim 1 , wherein the step of oscillating does not rotate the vial about the central vial axis.
3. The method of claim 1 , wherein the step of oscillating includes oscillating the vial at 1500 rpm or greater.
4. The method of claim 1 , wherein the step of oscillating includes oscillating the platform in a vertical motion.
5. The method of claim 1 , wherein the step of oscillating includes oscillating the platform back and forth along a distance of 1.25 inches.
6. The method of claim 1 , wherein the size of the vial is 740 ml or greater.
7. The method of claim 1 , wherein a diameter of each ball is 7 mm or greater.
8. The method of claim 1 , wherein the plurality of balls includes 10 or more.
9. The method of claim 1 , wherein the cylindrical sidewall of the vial has a diameter of 56 mm or greater.
10. The method of claim 9 , wherein the step of oscillating includes oscillating the platform back and forth along a distance of 3.2 cm.
11. The method of claim 1 , wherein the vial has a length between the end walls along the central vial axis of 82 mm or greater.
12. The method of claim 1 , wherein the step of loading the sample includes loading a substantially dry, non-liquid sample material into the vial.
13. A method of grinding large sample quantities using a bead beater homogenizer, the method comprising steps of:
loading a sample material into a vial having a diameter of 56 mm or greater having a chamber enclosed by two end walls and a cylindrical sidewall defining a central vial axis;
loading a plurality of balls into the vial with the sample material;
securing the vial to a movable platform of the homogenizer; and
oscillating the platform in a back-and-forth motion isolated to a movement axis along a distance of 3.2 cm, thereby causing the balls to move in a circular motion along the cylindrical sidewall, wherein the central vial axis is perpendicular to the movement axis.
14. A system comprising:
a cylindrical vial having a chamber enclosed by two end walls and a cylindrical sidewall defining a central vial axis, wherein the vial has a size of 500 ml or greater; and
a bead beater homogenizer for grinding large sample quantities comprising:
a movable platform;
a motor for oscillating the platform in a back-and-forth motion isolated to a movement axis, thereby causing rotation of the balls in a circular motion along the cylindrical sidewall;
a vial holder configured to hold the cylindrical vial such that the central vial axis is perpendicular to the movement axis; and
a clamp for securing the vial holder to the platform.
15. The system of claim 14 , further comprising a plurality of balls to be placed within the vial.
16. The system of claim 15 , wherein a diameter of each ball is 7 mm or greater.
17. The system of claim 14 , wherein the motor is configured to oscillate the vial without rotating the vial about the central vial axis.
18. The system of claim 14 , wherein the size of the vial is 740 ml or greater.
19. The system of claim 14 , wherein the motor is configured to oscillate the vial at 1500 rpm or greater.
20. The system of claim 14 , wherein the plurality of balls includes 10 or more.