Effective vaccination against porcine reproductive and respiratory syndrome (PRRS) virus prior to weaning
The invention provides isolated polynucleotide molecules that comprise a DNA sequence encoding an infectious RNA sequence encoding a genetically-modified North American PRRS virus, methods to make it and related polypeptides, polynucleotides, and various components. Vaccines comprising the genetically modified virus and polynucleotides and a diagnostic kit to distinguish between naturally infected and vaccinated animals are also provided.
1. A vaccine for protecting a porcine animal against infection by a PRRS virus, which vaccine comprises (a) a polynucleotide molecule of SEQ ID NO: 6 or a polynucleotide molecule that has at least 85% identity to SEQ ID NO: 6, wherein the protein encoded by ORF1a thereof has an amino acid sequence that contains:
(1) one, two, three, or four of:
an amino acid N within the amino acid sequence ANV (see SEQ ID NO: 9);
an amino acid N within the amino acid sequence HNA (see SEQ ID NO: 12);
an amino acid Y within the amino acid sequence WYL (see SEQ ID NO: 18); and
an amino acid H within the amino acid sequence VHG (see SEQ ID NO: 21); and
(2) one or both of
an amino acid T within the amino acid sequence ATD (see SEQ ID NO: 27); and
an amino acid C within the amino acid sequence MCQ (see SEQ ID NO: 33); and
(3) one or both of
an amino acid T within the amino acid sequence PTG (see SEQ ID NO: 36); and
an amino acid A within the amino acid sequence VAK (see SEQ ID NO: 39); and
(4) an amino acid N within the amino acid sequence FNP (see SEQ ID NO: 45); or
(b) a North American PRRS virus encoded by said polynucleotide molecule of (a) or,
(c) said polynucleotide molecule in the form of a plasmid, or
(d) a viral vector comprising said polynucleotide molecule, wherein the PRRS virus is able to elicit an effective immunoprotective response against infection by PRRS virus, in an amount effective to produce immunoprotection against infection, and a carrier suitable for veterinary use, and wherein said vaccine provides early and safe vaccination as early as when the piglet is 1 day of age, and wherein said vaccine provides a duration of immunity for up to 6 months.
2. The vaccine of claim 1 wherein onset of immunity is provided beginning at two weeks after vaccination.
3. A method for vaccinating a porcine animal against infection by a PRRS virus, comprising administering the vaccine of claim 1 to said porcine, wherein said porcine is between about 12 hours after birth and 2 weeks of age and wherein the duration of protective immunity provided is up to 6 months.
4. The method of claim 3 wherein protective immunity arises no later than about 14 days after vaccination.
5. The method of claim 4 , wherein protective immunity arises at Day 15 following vaccination on Day 1 of life, Day 21 following vaccination on Day 7 of life, or no later than about Day 28 following vaccination on Day 14 of life.