Methods of Genomic Evaluation in Livestock
The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning.
1 . A method of estimating a production value, a genotypic value or a breeding value of a non-human mammalian fetus comprising:
obtaining omics data comprising one or more features from one or more fetal amniocytes or fetal cells obtained in vivo;
calculating feature weights for the one or more features;
calculating a production value, a genotypic value or a breeding value of the fetus based on the calculated feature weights; and
selecting the fetus as a parent or to produce gametes based on the calculated production value, genotypic value or breeding value.
2 . The method of claim 1 , further comprising the step of producing offspring from the selected fetus.
3 . The method of claim 1 , further comprising the step of isolating the one or more fetal amniocytes from amniotic fluid.
4 . The method of claim 1 , further comprising the step of cloning the fetus using a fetal amniocyte or fetal cell obtained in vivo.
5 . The method of claim 1 , wherein the one or more fetal amniocytes comprise amniotic fluid-derived mesenchymal stem cells.
6 . The method of claim 1 , wherein the non-human mammalian fetus is a bovid.
7 . The method of claim 1 , wherein the step of obtaining omics data comprises obtaining DNA, RNA, a protein or a metabolite from the one or more fetal amniocytes or fetal cells, or detecting a protein or a metabolite in the one or more fetal amniocytes or fetal cells.
8 . The method of claim 7 , wherein the RNA is comprised of mRNA, pre-mRNA, tRNA, rRNA, ncRNA, lncRNA, miRNA, siRNA, snoRNA, piRNA, tsRNA or srRNA