Anti-galactofuranose antibodies for detecting and treating aspergillosis
It is provided novel anti-galactofuranose antibodies and their use for diagnosis of and/or treating aspergillosis, and for the design of chimeric antigen receptor T-cells, wherein single chain variable fragment of the antibodies, such as a heavy chain variable region or a light chain variable region, is fused via a spacer and a transmembrane domain to a signaling endodomain to generate an expression cassette that will be integrated into a T cell.
1. An antibody or a functional fragment thereof, comprising at least one of:
a) a heavy chain encoded by the DNA sequence consisting of SEQ ID NO: 5;
b) a heavy chain amino acid sequence consisting of SEQ ID NO: 6;
c) a light chain encoded by the DNA sequence consisting of SEQ ID NO: 7;
d) a light chain amino acid sequence consisting of SEQ ID NO: 8; and
e) a combination thereof.
2. The antibody or a functional fragment thereof of claim 1 , wherein said antibody is a humanized antibody, a monoclonal antibody or a polyclonal antibody.
3. The antibody or a functional fragment thereof of claim 1 , wherein said antibody is a mouse antibody, a goat antibody, a human antibody or a rabbit antibody.
4. The antibody or a functional fragment thereof of claim 1 , wherein said antibody comprises an epitope binding fragment selected from the group consisting of: Fv, F(ab′) and F(ab′) 2 .
5. The antibody or a functional fragment thereof of claim 1 , wherein said antibody comprises a fluorochrome or a labeling molecule.
6. The antibody or a functional fragment thereof of claim 5 , wherein said labeling molecule is biotin, peroxidase, alkaline phosphatase, an oligonucleotide labeling, a radiolabel, a metal, glucoamylase or B-galactosidase.
7. The antibody or a functional fragment thereof of claim 1 , wherein said antibody comprises the heavy chain encoded by the DNA sequence consisting of SEQ ID NO: 5; the heavy chain amino acid sequence consisting of SEQ ID NO: 6; the light chain encoded by the DNA sequence consisting of SEQ ID NO: 7; and the light chain amino acid sequence consisting of SEQ ID NO: 8, binds to a galactofuranose oligosaccharide.
8. The antibody or a functional fragment thereof of claim 7 , wherein said antibody detects a polysaccharide comprising a mono-, di-, tri-, or tetrasaccharides of galactofuranose.
9. The antibody or a functional fragment thereof of claim 7 , wherein said galactofuranose oligosaccharide is:
10. The antibody or a functional fragment thereof of claim 1 , said antibody is attached to a solid support.
11. A composition comprising the antibody or a functional fragment thereof of claim 1 and a carrier.
12. The antibody or a functional fragment thereof of claim 9 , wherein X=0 to 20.
13. The antibody or a functional fragment thereof of claim 9 , wherein X=0 to 10.
14. The antibody or a functional fragment thereof of claim 9 , wherein X=0 to 3.