Method for moving a processing vial between locations of an instrument
A method for moving a processing vial between locations of an instrument. The method includes the steps of dispensing a fluid into a processing vial with a disposable pipette tip frictionally fitted onto a probe of a pipettor, stripping the disposable pipette tip from the probe of the pipettor, and then engaging a cap in a frictional fit with the probe of the pipettor. While the cap is engaged in a frictional fit with the probe of the pipettor, coupling the cap to the processing vial to form a cap/vial assembly, where the cap seals the processing vial. The pipettor moves the cap/vial assembly from a first location of an instrument to a second location of the instrument. At the second location of the instrument, the cap/vial assembly is ejected from the probe of the pipettor, thereby depositing the cap/vial assembly at the second location.
1. A method for moving a fluid-containing processing vial between locations of an instrument, the method comprising the automated steps of:
(a) dispensing a fluid into a processing vial, the fluid being dispensed with a pipette tip engaged in a frictional fit with a probe of a pipettor;
(b) after step (a), stripping the pipette tip from the probe of the pipettor;
(c) inserting the probe of the pipettor into an open top end of a cap, thereby engaging the cap in a frictional fit;
(d) while the cap is engaged by the probe of the pipettor, inserting a closed lower end of the cap into an open top end of the processing vial to thereby form a cap/vial assembly, wherein the cap is coupled to the processing vial and the processing vial is sealed by the cap;
(e) with the pipettor, moving the cap/vial assembly from a first location of an instrument, where the cap/vial assembly is formed, to a second location of the instrument; and
(f) at the second location of the instrument, ejecting the cap/vial assembly from the probe of the pipettor, thereby depositing the cap/vial assembly at the second location.
2. The method of claim 1 , wherein the fluid dispensed into the processing vial in step (a) comprises a specimen.
3. The method of claim 2 , wherein the fluid dispensed into the processing vial in step (a) is an eluate.
4. The method of claim 2 , further comprising, prior to step (c), the step of dispensing a reaction fluid into the processing vial for performing a PCR reaction prior to forming the cap/vial assembly.
5. The method claim 4 , wherein the reaction fluid comprises a Taq NA polymerase, deoxynucleoside triphosphates, and magnesium chloride.
6. The method of claim 4 , further comprising, prior to step (c), the step of dispensing an oil into the processing vial prior to forming the cap/vial assembly.
7. The method of claim 1 , further comprising the step of dispensing an oil into the processing vial prior to forming the cap/vial assembly.
8. The method of claim 1 , wherein cap and vial of the cap/vial assembly are coupled together in an interlocking, snap-fit relationship.
9. The method of claim 8 , wherein the closed lower end of the cap defines a plug that fits into the open top end of the processing vial, thereby forming a frictional fit with the processing vial during step (d).
10. The method of claim 8 , wherein the processing vial comprises an open top end surrounded by a locking collar with lateral through-holes formed in the locking collar and a latch hook above each through-hole, and wherein the cap comprises a latch collar that extends about the cap and snaps beneath the latch hooks of the processing vial during step (d), thereby coupling the cap to the processing vial.
11. The method of claim 10 , wherein the closed lower end of the cap defines a plug that fits into the open top end of the processing vial, thereby forming a frictional fit with the processing vial during step (d).
12. The method of claim 11 , wherein the cap further comprises at least one seal ring extending about the lower portion of the cap below the latch collar.
13. The method of claim 10 , wherein the cap comprises an annular collar positioned between the open top end and the lower end of the cap, and wherein a bottom surface of the annular collar abuts a top surface of the locking collar when the cap/vial assembly is formed in step (d).
14. The method of claim 8 , wherein the cap comprises a latch collar with latch fingers and the processing vial comprises a lock collar surrounding an open top end of the processing vial, and wherein the latch fingers snap onto the lock collar of the processing vial during step (d).
15. The method of claim 1 , wherein each of the cap and the processing vial is formed from a plastic.
16. The method of claim 1 , wherein the cap is supported by a cap well of a tray during step (c) and the processing vial is supported by a processing vial well of the tray during step (d).
17. The method of claim 1 , wherein the second location of the instrument comprises a centrifuge, and wherein the cap/vial assembly is deposited into the centrifuge in step (f).
18. The method of claim 17 , further comprising the step of centrifuging the cap/vial assembly to remove air bubbles from a content of the processing vial.
19. The method of claim 18 , wherein the cap/vial assembly is deposited into the centrifuge at a first access port prior to the step of centrifuging the cap/vial assembly.
20. The method of claim 19 , further comprising, after the step of centrifuging the cap/vial assembly, the step of removing the cap/vial assembly from the centrifuge at a second access port, wherein the first and second access ports are at different locations.
21. The method of claim 20 , wherein the step of removing the cap/vial assembly from the centrifuge comprises engaging the cap of the cap/vial assembly in a frictional fit with a probe of a vial transfer arm.
22. The method of claim 21 , further comprising, while the cap of the cap/vial assembly is engaged in a frictional fit with the vial transfer arm, moving the cap/vial assembly from the second access port of the centrifuge to a thermal cycler.
23. The method of claim 22 , further comprising the step of stripping the cap/vial assembly from the vial transfer arm, thereby depositing the cap/vial assembly into a well of the thermal cycler.
24. The method of claim 21 , wherein the pipettor is unable to access the second access port and the vial transfer arm is unable to access the first access port.
25. The method of claim 1 , wherein the second location of the instrument comprises a thermal cycler, and wherein the cap/vial assembly is deposited into a well of the thermal cycler in step (f).
26. The method of claim 25 , wherein step (e) further comprises, with the pipettor, moving the cap/vial assembly from the first location to a centrifuge prior to moving the cap/vial assembly to the thermal cycler.
27. The method of claim 1 , wherein step (f) comprises stripping the cap/vial assembly from the probe of the pipettor with a stripping device.
28. The method of claim 27 , wherein the stripping device engages a rim of the cap.
29. The method of claim 28 , wherein the stripping device is a strip sleeve on the probe of the pipettor.
30. The method of claim 1 , further comprising the step of detecting the presence or amount of an analyte suspected of being present in the fluid.