IP Library › Patent Application 16834141
Patent Application
App. No. 16/834,141

DEGENERATE OLIGONUCLEOTIDES AND THEIR USES

Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US None
App. No.
16/834,141
Abstract

The present invention provides a plurality of oligonucleotides comprising a semi-random sequence, wherein the semi-random sequence comprises degenerate nucleotides that are substantially non-complementary. Also provided are methods for using the plurality of oligonucleotides to amplify a population of target nucleic acids.

Claims (4)

1 . A plurality of degenerate oligonucleotides, each oligonucleotide having a sequence selected from KNNNKNKNK, NKNNKNNKK, or NNNKNKKNK, wherein

N is a 4-fold degenerate nucleotide selected from adenosine (A), cytidine (C), guanosine (G), or thymidine/uridine (T/U); and

K is G or T/U.

2 . The plurality of degenerate oligonucleotides of claim 1 , wherein each oligonucleotide further comprises a sequence of non-degenerate nucleotides at the 5′ end, the non-degenerate sequence being constant among the plurality of oligonucleotides, and the constant non-degenerate sequence being about 14 nucleotides to about 24 nucleotides in length.