Coiled coil immunoglobulin fusion proteins and compositions thereof
Disclosed herein are immunoglobulin fusion proteins comprising a first antibody region, a first therapeutic agent, and a first connecting peptide; wherein the first therapeutic agent is attached to the first antibody region by the connecting peptide; and wherein the connecting peptide does not comprise a region having beta strand secondary structure. The connecting peptide may comprise an extender peptide. The extender peptide may have an alpha helical secondary structure. The connecting peptide may comprise a linker peptide. The linker peptide may not comprise any secondary structure. Also disclosed herein are compositions comprising the immunoglobulin fusion proteins and methods for using the immunoglobulin fusion proteins for the treatment or prevention of a disease or condition in a subject.
1. An immunoglobulin fusion protein comprising a human or humanized antibody domain and a first extender fusion region positioned within the antibody domain, wherein the first extender fusion region comprises a therapeutic agent positioned between a first extender peptide and a second extender peptide, the first extender peptide comprising (AKLAALK)n (SEQ ID NO: 148) where n is from 1 to 5, and the second extender peptide comprising (ELAALEA)m (SEQ ID NO: 158) where m is from 1 to 5.
2. The immunoglobulin fusion protein of claim 1 , wherein n is from 1 to 3.
3. The immunoglobulin fusion protein of claim 1 , wherein n is 2.
4. The immunoglobulin fusion protein of claim 1 , wherein m is from 1 to 3.
5. The immunoglobulin fusion protein of claim 1 , wherein m is 2.
6. The immunoglobulin fusion protein of claim 1 , wherein the antibody domain comprises a heavy chain and a light chain, and the antibody domain comprises an antibody variable domain, and the first extender fusion region is positioned within the antibody variable domain.
7. The immunoglobulin fusion protein of claim 6 , wherein the first extender fusion region is positioned within a CDR of the antibody variable domain.
8. The immunoglobulin fusion protein of claim 6 , wherein the first extender fusion region is positioned within a CDR3 of the antibody variable domain.
9. The immunoglobulin fusion protein of claim 6 , wherein the first extender fusion region is positioned within the CDR3 of the heavy chain of the antibody variable domain.
10. The immunoglobulin fusion protein of claim 6 , wherein the first extender fusion region is positioned within the CDR3 of the light chain of the antibody variable domain.
11. The immunoglobulin fusion protein of claim 6 , wherein the first extender fusion region replaces a portion of the antibody variable domain.