Nucleic acids encoding antibodies specifically binding to MASP-3
The present invention relates to MASP-3 inhibitory antibodies and compositions comprising such antibodies for use in inhibiting the adverse effects of MASP-3 dependent complement activation.
1. A cloning or expression vector comprising a nucleic acid encoding complementarity determining regions (CDRs) of heavy and light chain variable regions of an antibody, or antigen-binding fragment thereof, that binds to human MASP-3, wherein the heavy chain variable region comprises the amino acid sequence set forth as SEQ ID NO:254 or SEQ ID NO:255 and the light chain variable region comprises the amino acid sequence set forth as SEQ ID NO:45, SEQ ID NO:256 or SEQ ID NO:280.
2. The cloning or expression vector of claim 1 , wherein the heavy chain variable region comprises SEQ ID NO:254 and the light chain variable region comprises SEQ ID NO:45.
3. The cloning or expression vector of claim 1 , wherein the heavy chain variable region comprises SEQ ID NO:254 and the light chain variable region comprises SEQ ID NO:256.
4. The cloning or expression vector of claim 1 , wherein the heavy chain variable region comprises SEQ ID NO:254 and the light chain variable region comprises SEQ ID NO:280.
5. The cloning or expression vector of claim 1 , wherein the heavy chain variable region comprises SEQ ID NO:255 and the light chain variable region comprises SEQ ID NO:45.
6. The cloning or expression vector of claim 1 , wherein the heavy chain variable region comprises SEQ ID NO:255 and the light chain variable region comprises SEQ ID NO:256.
7. The cloning or expression vector of claim 1 , wherein the heavy chain variable region comprises SEQ ID NO:255 and the light chain variable region comprises SEQ ID NO:280.
8. A cell comprising the cloning or expression vector according to claim 1 .
9. A cell comprising:
(a) a nucleic acid encoding complementarity determining regions (CDRs) of a heavy chain variable region of an antibody, or antigen-binding fragment thereof, that binds to human MASP-3, wherein the heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:255; and
(b) a nucleic acid encoding complementarity regions (CDRs) of a light chain variable region of an antibody, or antigen-binding fragment thereof, that binds to human MASP-3, wherein the light chain variable region comprises the amino acid sequence set forth in SEQ ID NO:280.
10. The cell of claim 9 , wherein the nucleic acid according to (a) and the nucleic acid according to (b) are included in an expression vector in the cell.
11. The cell of claim 9 , wherein the nucleic acid according to (a) and the nucleic acid according to (b) are included in different expression vectors in the cell.
12. A method for producing an antibody, or antigen-binding fragment thereof, that binds to human MASP-3, the method comprising culturing the cell of claim 8 under conditions and for a time sufficient to allow expression by the cell of the antibody, or antigen-binding fragment thereof, encoded by the nucleic acid.
13. The method of claim 12 , further comprising isolating the antibody, or antigen-binding fragment thereof.