Engineered enzymes
The present disclosure provides engineered RNA-guided enzymes for editing live cells.
1. A cocktail of engineered nucleic acid-guided nucleases, wherein one or more nucleases in the cocktail has an altered fidelity relative to the nucleic acid-guided nuclease having the sequence of SEQ ID No. 1 and has a sequence comprising any of SEQ ID Nos. 4-7, and wherein one or more nucleases has editing activity in yeast different than the nucleic acid-guided nuclease having the sequence of SEQ ID No. 1 and has a sequence comprising any of SEQ ID Nos. 69-78 or 79-86.
2. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 69.
3. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 70.
4. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 71.
5. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 72.
6. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 73.
7. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 74.
8. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 75.
9. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 76.
10. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 77.
11. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 78.
12. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 79.
13. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 80.
14. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 81.
15. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 82.
16. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 83.
17. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 84.
18. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 85.
19. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 86.
20. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 4.
21. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 5.
22. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 6.
23. The cocktail of engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 7.