Immunogenic peptides and their use in immune disorders
The present invention provides novel peptides and homologues thereof. The peptides of the invention comprise (i) a T-cell epitope of an antigen (self or non-self) with a potential to trigger an immune reaction presented by a class II major histocompatibility complex (MHC) determinant and recognised by CD4+ T cell more specifically of an allergen or auto-antigen, coupled, optionally through the use of a linker to (ii) an amino acid sequence having a reducing activity, such as a thioreductase sequence. The peptides of the invention have been shown to be useful a medicine, more in particular for the prevention or treatment of immune disorders, more specifically of allergic disorders or autoimmune diseases. The present invention thus provides for the use of said peptides for the manufacture of a medicament for the prevention or treatment of an immune disorder and further provides for methods of treatment or preventing immune disorders by using said peptides. The present invention also provides for compositions comprising said peptides.
1. A method for treating an allergic condition in an individual, comprising administering to said individual an isolated immunogenic peptide with a length of at most 75 amino acids, wherein said immunogenic peptide comprises:
a T cell epitope sequence of an antigenic protein, wherein said antigenic protein is an allergen and wherein said T cell epitope sequence is an epitope sequence recognized by WIC class II molecules, and
a [CST]-(X)2-C or C-X(2)-[CST] thioredox sequence motif wherein X is any amino acid,
said thioredox sequence motif being either adjacent to said epitope sequence, or separated from said epitope sequence by a linker of at most 7 amino acids, wherein said thioredox sequence motif does not naturally occur within a region of 11 amino acids N-terminally or C-terminally of the T cell epitope sequence in said antigenic protein.
2. The method according to claim 1 , wherein said T cell epitope sequence has a length of 8 to 16 amino acids.
3. The method according to claim 1 , wherein said thioredox sequence motif is separated from said T cell epitope sequence by a linker of at most 4 amino acids.
4. The method according to claim 1 , wherein said immunogenic peptide has a length between 12 and 50 amino acids.
5. The method according to claim 1 , wherein at least one X in the thioredox sequence motif is Gly, Ala, Ser, Thr, His or Pro.
6. The method according to claim 1 , wherein said thioredox sequence motif is positioned N-terminally of said T cell epitope sequence.
7. The method according to claim 1 , wherein said allergen is selected from the group consisting of: food allergens, house dust mites allergens, allergens from insects, allergens from pollen, allergens from animals, allergens from fungi, and occupational allergens.
8. The method according to claim 7 , wherein said allergen is selected from the group consisting of Betula Bet v1 allergen, Bovine beta-lactoglobulin and Der p1.