Hybridization compositions and methods for making and using compositions
The present disclosure relates to hybridization buffers for use in hybridization, for example, for use in in situ hybridization (ISH). Hybridization buffers and compositions comprising a sulfone solvent and polyvinyl sulfonic acid or a salt thereof, and methods of making and using, the same are disclosed.
1. An in situ hybridization buffer comprising:
a sulfone solvent in a concentration from 5 to 30% (v/v); and
a sulfonic acid polymer of formula (I), (Ia), (II), (IIa) or (III):
or a salt of any one of Formulas (I), (Ia), (II), (IIa) or (III), wherein: R 1 is hydrogen or C 1-6 aliphatic; L, when present, is a covalent bond or C 1-6 aliphatic, R 2 , when present, is a covalent bond, C 1-6 aliphatic, phenyl, or a 6-membered heteroaryl ring having 1-3 nitrogens, wherein: R 2 is substituted with 1 or 2-SO 3 H groups or a salt thereof, provided that when R 2 is a covalent bond, only 1-SO 3 H group or a salt thereof is present, and R 2 is optionally substituted with 1-2 groups independently selected from —-R 3 , halogen, —CN, —NO 2 , —OR 3 , —N(R 3 ) 2 , and —SR 3 , wherein each R 3 is independently hydrogen or a C 1-6 alkyl group; and n is an integer greater than 10; and
wherein the sulfonic acid polymer or salt thereof is present in a concentration from 5 to 50% (w/v); and
wherein the hybridization buffer is homogeneous without phase separation after storage at 2-8° C. for 2-24 months.
2. The hybridization buffer of claim 1 , wherein R 1 is hydrogen.
3. The hybridization buffer of claim 1 , wherein R 1 is C 1-6 aliphatic.
4. The hybridization buffer of claim 1 , wherein L is a covalent bond.
5. The hybridization buffer of claim 1 , wherein L is C 1-6 aliphatic.
6. The hybridization buffer of claim 1 , wherein L is methylene.
7. The hybridization buffer of claim 1 , further comprising water.
8. The hybridization buffer of claim 1 , wherein the sulfonic acid polymer or salt thereof is polyvinylsulfonic acid or a salt thereof.
9. The hybridization buffer of claim 1 , wherein the concentration of the sulfone solvent is from 10% to 20% (v/v).
10. The hybridization buffer of claim 1 , wherein the sulfone solvent is selected from the group consisting of dimethyl sulfone, diphenyl sulfone, methyl phenyl sulfone, tetramethylene sulfone (sulfolane), and mixtures thereof.
11. The hybridization buffer of claim 1 , wherein the sulfone solvent is sulfolane.
12. The hybridization buffer of claim 1 , wherein the concentration of the sulfonic acid polymer or salt thereof is from 20% to 40% (w/v).
13. The hybridization buffer of claim 1 , further comprising dextran sulfate in concentration no greater than 20% (w/v).
14. The hybridization buffer of claim 1 , wherein the hybridization buffer has a viscosity at 25° C. of 60 mPa·s, as measured by microviscometry.
15. The hybridization buffer of claim 1 , wherein the hybridization buffer is stable at 2-8° C. for 2-24 months.
16. The hybridization buffer of claim 1 , further comprising NaCl and 3-(N-morpholino) propanesulfonic acid (MOPS).
17. The hybridization buffer claim 1 , further comprising:
an accelerating agent in a concentration no greater than 20% (w/v), wherein
the sulfone solvent is sulfolane in a concentration of 10% (v/v),
the sulfonic acid polymer or salt thereof is in a concentration of 30% (w/v).