Molecular markers linked to disease resistance in soybean
The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having a Rps1 resistance allele and resistance to Phytophthora sojae . A soybean plant, part thereof and/or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided.
1. A method of producing a Glycine max plant that has improved resistance to Phytophthora as compared to a control plant, the method comprising the steps of:
(a) isolating a nucleic acid from a Glycine max plant;
(b) detecting in the nucleic acid of (a) the presence of a genetic marker that is associated with improved resistance to Phytophthora and is associated with Rps1c, wherein said genetic marker comprises SY2726DQ (SEQ ID NO: 5) and further comprises an A allele at nucleotide 251 of SEQ ID NO:5 (SY2726DQ) or is a genetic marker linked thereto, wherein said genetic marker linked thereto comprises at least one of an A allele at nucleotide 251 of SEQ ID NO: 1 (SY2723AQ), a C allele at nucleotide 251 of SEQ ID NO: 3 (SY2724AQ), a G allele at nucleotide 251 of SEQ ID NO: 4 (SY2726BQ), an A allele at nucleotide 251 of SEQ ID NO: 6 (SY2724BQ), an A allele at nucleotide 251 of SEQ ID NO: 7 (SY2724CQ), and a G allele at nucleotide 251 of SEQ ID NO: 8 (SY2725AQ);
(c) selecting a first Glycine max plant based on the presence of the marker associated with improved resistance to Phytophthora in (b);
(d) crossing a second Glycine max plant with the first Glycine max plant of (c) wherein, said second Glycine max plant does not comprise in its genome the marker associated with improved resistance to Phytophthora in (c);
(e) producing seed from the crossing of (d); and
(f) selecting a Glycine max plant, grown from the seed of (e), that has improved resistance to Phytophthora and comprises the genetic marker associated with improved resistance to Phytophthora and associated with Rps1c.
2. The method of claim 1 , wherein Phytophthora is Phytophthora sojae.
3. The method of claim 1 , wherein the detecting comprises detecting the presence of the genetic marker comprising the A allele at nucleotide 251 of SEQ ID NO: 5 or an allelic form of said genetic marker linked thereto.
4. The method of claim 1 , wherein the second Glycine max plant is an elite soybean line.
5. The method of claim 1 , wherein the second Glycine max plant displays less resistance to Phytophthora as compared to the first Glycine max plant.
6. The method of claim 1 further comprising the step of backcrossing the plants produced in (e).