PEPTIDOMIMETIC AGENTS, SYNTHESIS AND USES THEREOF
Compounds for use in synthesis of peptidomimetic agents; synthesis of peptidomimetic agents; peptidomimetic diagnostic and therapeutic agents; and uses of the compounds and peptidomimetic agents in drug discovery, diagnosis, prevention and treatment of diseases are described.
1 . A compound of Formula (IA):
wherein
X is imidazolyl or benzotriazolyl, and wherein
(i) A and R are connected and form a side chain of proline, or
(ii) A is hydrogen, or a protecting group comprising phthalimidyl, tert-butoxycarbonyl, 9-fluorenylmethoxycarbonyl, 2-(3,5-dimethoxyphenyl)propan-2-yloxycarbonyl; and R is selected from the group consisting of side chain radicals of aspartic acid, phenylalanine, alanine, histidine, glutamic acid, tryptophan, valine, leucine, lysine, methionine, tyrosine, isoleucine, arginine, glycine, asparagine, serine, cysteine, serine, threonine, and glutamine.
2 . The compound of claim 1 , wherein R is substituted with one or more of the following: a halogen, a C 1 -C 6 alkyl, hydroxyl, —COOH, —COH, methoxyl, ethoxyl, propoxyl, a C 1 -C 6 haloalkyl or a protecting group.
3 . The compound of claim 1 , wherein R is unsubstituted.
4 . The compound of claim 1 , wherein X is imidazolyl.
5 . The compound of claim 4 , which is selected from the group consisting of
wherein PG is selected from the group consisting of H, phthalimidyl, tert-butoxycarbonyl, 9-fluorenylmethoxycarbonyl, and 2-(3,5-dimethoxyphenyl)propan-2-yloxycarbonyl.
6 . The compound of claim 1 , wherein X is benzotriazolyl.
7 . The compound of claim 6 , wherein the compound is selected from the group consisting of
wherein PG is selected from the group consisting of H, phthalimidyl, tert-butoxycarbonyl, 9-fluorenylmethoxycarbonyl, and 2-(3,5-dimethoxyphenyl)propan-2-yloxycarbonyl.
8 . A method of synthesis of an azapeptide comprising coupling a compound according to claim 1 to an amino acid or an aza-amino acid, wherein the azapeptide is a compound of Formula (V):
or a pharmaceutically acceptable salt thereof, wherein
B is independently selected from the group consisting of hydrogen, —NH 2 , —NNH 2 , —CONH 2 , —COOH, —COH, —COC 1 -C 4 alkyl, —COC 1 -C 4 haloalkyl, —OH, an amino acid, an aza amino acid, a 2 to 60-mer peptide, a 2 to 60-mer aza peptide, a 2 to 60-mer azatide;
D is independently selected from the group consisting of —OH, —NH 2 , —NNH 2 , —CONH 2 , —COOH, —COH, —COC 1 -C 4 alkyl, —COC 1 -C 4 haloalkyl, an amino acid, an aza amino acid, a 2 to 60-mer peptide, a 2 to 60-mer aza peptide, a 2 to 60-mer azatide; and
R is independently selected from the group consisting of unsubstituted and substituted side chain radicals of aspartic acid, phenylalanine, alanine, histidine, glutamic acid, tryptophan, valine, leucine, lysine, methionine, tyrosine, isoleucine, arginine, glycine, asparagine, serine, and glutamine.
9 . The method of claim 8 , wherein the coupling is during a solid phase peptide synthesis.
10 . The method of claim 8 , wherein the coupling is during a liquid phase peptide synthesis.
11 . The method of claim 9 , comprising activating the compound according to claim 1 prior to the coupling.
12 . The method of claim 11 , wherein the activating is with Mel.
13 . The method of claim 11 , wherein the activating is with DIPEA.
14 . The method of claim 10 , comprising activating the compound according to claim 1 prior to the coupling.
15 . The method of claim 14 , wherein the activating is with Mel.
16 . The method of claim 14 , wherein the activating is with DIPEA.
17 . The method of claim 8 , wherein the compound of Formula (V) is a di-azatide, a tri-azatide, a tetra-azapeptide, or a tetra-azatide.
18 . The method of claim 9 , wherein the compound of Formula (V) is produced in a yield of at least about 50%.
19 . The method of claim 10 , wherein the compound of Formula (V) is produced in a yield of at least about 50%.
20 . A method of treating a disorder comprising administering a therapeutically effective amount of the azapeptide prepared according to claim 9 or claim 10 to a subject in need thereof.