Compositions and methods for controlling plant pests
Novel insecticidal proteins that are toxic to lepidopteran pests are disclosed. The DNA encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or the insecticidal proteins alone or in combination with an appropriate agricultural carrier can be used to control lepidopteran pests in various environments.
1. A nucleic acid molecule comprising a synthetic nucleotide sequence that has codons optimized for expression in a transgenic organism, wherein the nucleotide sequence encodes SEQ ID NO:41.
2. The nucleic acid molecule of claim 1 , wherein the nucleotide sequence comprises SEQ ID NO:21.
3. A chimeric gene comprising a heterologous promoter operably linked to the nucleic acid molecule of claim 1 .
4. The chimeric gene of claim 3 , wherein the heterologous promoter is a plant expressible promoter.
5. The chimeric gene of claim 4 , wherein the plant expressible promoter is selected from the group of promoters consisting of ubiquitin, cestrum yellow virus, corn TrpA, OsMADS 6, maize H3 histone, corn sucrose synthetase 1, corn alcohol dehydrogenase 1, corn light harvesting complex, corn heat shock protein, maize mtl, pea small subunit RuBP carboxylase, rice actin, rice cyclophilin, Ti plasmid mannopine synthase, Ti plasmid nopaline synthase, petunia chalcone isomerase, bean glycine rich protein 1, potato patatin, lectin, CaMV 35S and S-E9 small subunit RuBP carboxylase promoter.
6. A recombinant vector comprising the nucleic acid molecule of claim 1 .
7. A transgenic bacterial or plant cell comprising the recombinant vector of claim 6 .
8. The transgenic bacterial cell of claim 7 , wherein the bacterial cell is in the genus Bacillus, Clostridium, Xenorhabdus, Photorhabdus, Pasteuria, Escherichia, Pseudomonas, Erwinia, Serratia, Klebsiella, Salmonella, Pasteurella, Xanthomonas, Streptomyces, Rhizobium, Rhodopseudomonas, Methylophilius, Agrobacterium, Acetobacter, Lactobacillus, Arthrobacter, Azotobacter, Leuconostoc , or Alcaligenes.
9. The transgenic plant cell of claim 7 , wherein the plant cell is a) a dicot plant cell; or b) a monocot plant cell; or c) a dicot plant cell selected from the group consisting of a soybean cell, sunflower cell, tomato cell, cole crop cell, cotton cell, sugar beet cell and tobacco cell; or d) a monocot plant cell selected from the group consisting of a barley cell, maize cell, oat cell, rice cell, sorghum cell, sugar cane cell and wheat cell.
10. A transgenic plant comprising the transgenic plant cell of claim 9 , wherein the plant is a) a dicot plant; or b) a monocot plant; or c) a dicot plant selected from the group consisting of a soybean plant, sunflower plant, tomato plant, cole crop plant, cotton plant, sugar beet plant and a tobacco plant; or d) a monocot plant selected from the group consisting of a barley plant, maize plant, oat plant, rice plant, sorghum plant, sugar cane plant and a wheat plant.
11. A transgenic seed of the transgenic plant of claim 10 , wherein the transgenic seed comprises the nucleic acid molecule.
12. A method of producing an insect-resistant transgenic plant, comprising:
introducing into a plant the chimeric gene of claim 3 , wherein the Cry protein is expressed in the plant, thereby producing an insect-resistant transgenic plant.
13. The method of claim 12 , wherein the introducing step is achieved by a) transforming the plant; or b) crossing a first plant comprising the chimeric gene with a different second plant.
14. The method of claim 13 , wherein the plant is a maize plant.
15. A method of controlling a CrylAb-resistant lepidopteran insect comprising contacting the insect with the transgenic plant of claim 10 .