SPRAY-DRIED COMPOSITION COMPRISING BETA-GALACTOSIDASE HAVING TRANSGALACTOSYLATING ACTIVITY IN COMBINATION WITH MALTODEXTRIN AND/OR NACL AND APPLICATION OF THE COMPOSITION
A spray-dried composition comprising an enzyme which is a β-galactosidase having transgalactosylating activity and a maltodextrin and/or sodium chloride.
1 . A spray-dried composition comprising a polypeptide which is a β-galactosidase having transgalactosylating activity and a maltodextrin.
2 . A spray-dried composition comprising a polypeptide which is a β-galactosidase having transgalactosylating activity and sodium chloride.
3 . The spray-dried composition according to claim 1 or claim 2 comprising a polypeptide which is a β-galactosidase having transgalactosylating activity, a maltodextrin and sodium chloride.
4 . The composition of any preceding claim, wherein the polypeptide is an enzyme which is classified in Enzyme Classification (E.C.) 3.2.1.23.
5 . The composition of any preceding claim, wherein the polypeptide has a ratio of transgalactosylating activity: β-galactosidase activity of at least 0.5, at least 1, at least 2, at least 2.5, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, or at least 12 at or above a concentration of 3% w/w initial lactose concentration.
6 . The composition of any one of claims 1 to 4 , wherein the polypeptide has a transgalactosylating activity such that more than 20%, more than 30%, more than 40%, up to 50% of the initial lactose is transgalactosylated as measured at a concentration of 100 ppm in a milk-based assay at 37° C. and 5 w/w % lactose after 15, 30 or 180 such as 180 minutes of reaction.
7 . The composition of any one of claims 1 to 4 , wherein the polypeptide has a β-galactosidase activity such that less than 80%, less than 70%, less than 60%, less than 50%, less than 40%, less than 30%, less than 20% of the lactose has been hydrolysed as measured at a concentration of 100 ppm in a milk-based assay at 37° C. and 5 w/w % lactose after 15, 30 or 180 such as 180 minutes of reaction.
8 . The composition of any one of claims 5 to 7 wherein the activity is retained for a period of at least 1, at least 2, at least 3, at least 4, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 18, or at least 24 months.
9 . The composition of any preceding claim, wherein the polypeptide having a transgalactosylating activity is selected from the group consisting of:
a. a polypeptide comprising an amino acid sequence having at least 90% sequence identity with SEQ ID NO: 1, wherein said polypeptide consists of at most 980 amino acid residues,
b. a polypeptide comprising an amino acid sequence having at least 97% sequence identity with SEQ ID NO: 2, wherein said polypeptide consists of at most 975 amino acid residues,
c. a polypeptide comprising an amino acid sequence having at least 96.5% sequence identity with SEQ ID NO: 3, wherein said polypeptide consists of at most 1300 amino acid residues,
d. a polypeptide encoded by a polynucleotide that hybridizes under at least low stringency conditions with i) the nucleic acid sequence comprised in SEQ ID NO: 9, 10, 11, 12 or 13 encoding the polypeptide of SEQ ID NO: 1, 2, 3, 4 or 5; or ii) the complementary strand of i),
e. a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% identity to the nucleotide sequence encoding for the polypeptide of SEQ ID NO: 1, 2, 3, 4 or 5 or the nucleotide sequence comprised in SEQ ID NO: 9, 10, 11, 12 or 13 encoding a mature polypeptide, and
f. a polypeptide comprising a deletion, insertion and/or conservative substitution of one or more amino acid residues of SEQ ID NO: 1, 2, 3, 4 or 5.
10 . The composition according to any preceding claim wherein the polypeptide having transgalactosylating activity is selected from the group consisting of:
a. a polypeptide comprising an amino acid sequence having at least 96.5% sequence identity with SEQ ID NO: 3, wherein said polypeptide consists of at most 1300 amino acid residues,
b. a polypeptide comprising an amino acid sequence having at least 90% sequence identity with SEQ ID NO: 1, wherein said polypeptide consists of at most 980 amino acid residues,
c. a polypeptide encoded by a polynucleotide that hybridizes under at least low stringency conditions with i) the nucleic acid sequence comprised in SEQ ID NO: 9, 10, 11, 12 or 13 encoding the polypeptide of SEQ ID NO: 1, 2, 3, 4, or 5; or ii) the complementary strand of i),
d. a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% identity to the nucleotide sequence encoding for the polypeptide of SEQ ID NO: 1, 2, 3, 4 or 5 or the nucleotide sequence comprised in SEQ ID NO: 9, 10, 11, 12 or 13 encoding a mature polypeptide, and
e. a polypeptide comprising a deletion, insertion and/or conservative substitution of one or more amino acid residues of SEQ ID NO: 1, 2, 3, 4 or 5.
11 . The composition according to any preceding claim, wherein the polypeptide having transgalactosylating activity comprises or consists of the amino acid sequence of SEQ ID NO:1, 2, 3, 4 or 5.
12 . The composition according to any preceding claim, wherein the composition contains 0.1 wt % or less polyol.
13 . The composition according to any preceding claim, wherein the composition further contains potato starch.
14 . A method of spray drying a composition comprising:
a. introducing a composition into a spray drying apparatus, wherein the composition comprises an enzyme as defined in any one of claims 1 to 13 and a maltodextrin; and
b. spray drying the composition to produce particles.
15 . A method of spray drying a composition comprising:
a. introducing a composition into a spray drying apparatus, wherein the composition comprises an enzyme as defined in any one of claims 1 to 13 and sodium chloride; and
b. spray drying the composition to produce particles.
16 . A method for producing a food product by treating a substrate comprising lactose with a composition as defined in any one of claims 1 to 13 .
17 . The method according to claim 15 for producing a dairy product by treating a milk-based substrate comprising lactose with a composition as defined in any one of claims 1 to 13 .
18 . A process for producing galacto-oligosaccharides, comprising contacting the composition of any one of claims 1 to 13 with a milk-based solution comprising lactose.