Microbial production of fatty diols
The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.
1. A method for producing a 1,3 fatty diol comprising:
(i) culturing a recombinant microorganism in a fermentation broth that comprises a simple carbon source, wherein said recombinant microorganism expresses;
a nucleic acid sequences encoding a thioesterase having the classification EC 3.1.2.-, EC 3.1.1.5, or EC 3.1.2.14, wherein the thioesterase uses 3-hydroxyacyl-ACP as a substrate; and
a nucleic acid sequence encoding a carboxylic acid reductase (CAR) having the classification EC 1.2.99.6; and
(ii) isolating the 1.3 fatty diol from said fermentation broth.
2. The method of claim 1 , wherein said 1,3 fatty diol is produced in vivo.
3. The method of claim 1 , wherein said 1,3 fatty diol is selected from the group consisting of a C 5 1,3 fatty diol, a C 6 1,3 fatty diol, a C 7 1,3 fatty diol, a C 8 1,3 fatty diol, a C 9 1,3 fatty diol, a C 10 1,3 fatty diol, a C 11 1,3 fatty diol, a C 12 1,3 fatty diol, a C 13 1,3 fatty diol, a C 14 1,3 fatty diol, a C 15 1,3 fatty diol, a C 16 1,3 fatty diol, a C 17 1,3 fatty diol, a C 18 1,3 fatty diol, and a C 19 1,3 fatty diol.
4. The method of claim 1 , wherein the recombinant microorganism further expresses a nucleic acid sequence encoding an alcohol dehydrogenase having the classification EC 1.1.1.-.
5. The method of claim 1 , wherein said simple carbon source is derived from a renewable feedstock.
6. The method of claim 1 , wherein:
the thioesterase is FatB1; and
the carboxylic acid reductase (CAR) is selected from the group consisting of CarB from Mycobacterium smegmatis , CarA from Mycobacterium smegmatis , FadD9 from Mycobacterium smegmatis , CAR from Mycobacterium genavense , CAR from Nocardia iowensis , and CAR from Nocardia brasiliensis.
7. The method of claim 4 , wherein said alcohol dehydrogenase is alrA.
8. The method of claim 1 , wherein the recombinant microorganism is a recombinant bacterium.
9. The method of claim 8 , wherein the recombinant bacterium is Escherichia coli ( E. coli ).