B*44 RESTRICTED PEPTIDES FOR USE IN IMMUNOTHERAPY AGAINST CANCERS AND RELATED METHODS
The present invention relates to peptides, proteins, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated T-cell peptide epitopes, alone or in combination with other tumor-associated peptides that can for example serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses, or to stimulate T cells ex vivo and transfer into patients. Peptides bound to molecules of the major histocompatibility complex (MHC), or peptides as such, can also be targets of antibodies, soluble T-cell receptors, and other binding molecules.
1 . A method of treating a patient who has cancer, comprising administering to said patient a population of activated T cells that kill cancer cells that present a peptide consisting of the amino acid sequence of SEQ ID NO: 41, wherein said cancer is head and neck squamous cell carcinoma or esophageal cancer.
2 . The method of claim 1 , wherein the T cells are autologous to the patient.
3 . The method of claim 1 , wherein the T cells are obtained from a healthy donor.
4 . The method of claim 1 , wherein the T cells are obtained from tumor infiltrating lymphocytes or peripheral blood mononuclear cells.
5 . The method of claim 1 , further comprising administering to said patient an adjuvant selected from anti-CD40 antibody, imiquimod, resiquimod, GM-CSF, cyclophosphamide, sunitinib, bevacizumab, interferon-alpha, interferon-beta, CpG oligonucleotides and derivatives, poly-(I:C) and derivatives, RNA, sildenafil, particulate formulations with poly(lactide co-glycolide) (PLG), virosomes, interleukin (IL)-1, IL-2, IL-4, IL-7, IL-12, IL-13, IL-15, IL-21, and IL-23.
6 . The method of claim 1 , wherein the activated T cells are cytotoxic T cells produced by contacting T cells with an antigen presenting cell that expresses the peptide in a complex with an MHC class I molecule on the surface of the antigen presenting cell, for a period of time sufficient to activate said T cell.
7 . The method of claim 6 , wherein the antigen presenting cell is infected with a recombinant virus expressing the peptide.
8 . The method of claim 1 , wherein the cancer is head and neck squamous cell carcinoma.
9 . The method of claim 1 , wherein the cancer is esophageal cancer.
10 . The method of claim 5 , wherein said adjuvant comprises IL-21.
11 . A method of eliciting an immune response in a patient who has cancer, comprising administering to said patient a population of activated T cells that kill cancer cells that present a peptide consisting of the amino acid sequence of SEQ ID NO: 41, wherein said cancer is head and neck squamous cell carcinoma or esophageal cancer.
12 . The method of claim 11 , wherein the T cells are autologous to the patient.
13 . The method of claim 11 , wherein the T cells are obtained from a healthy donor.
14 . The method of claim 11 , wherein the T cells are obtained from tumor infiltrating lymphocytes or peripheral blood mononuclear cells.
15 . The method of claim 11 , further comprising administering to said patient an adjuvant selected from anti-CD40 antibody, imiquimod, resiquimod, GM-CSF, cyclophosphamide, sunitinib, bevacizumab, interferon-alpha, interferon-beta, CpG oligonucleotides and derivatives, poly-(I:C) and derivatives, RNA, sildenafil, particulate formulations with poly(lactide co-glycolide) (PLG), virosomes, interleukin (IL)-1, IL-2, IL-4, IL-7, IL-12, IL-13, IL-15, IL-21, and IL-23.
16 . The method of claim 11 , wherein the activated T cells are cytotoxic T cells produced by contacting T cells with an antigen presenting cell that expresses the peptide in a complex with an MHC class I molecule on the surface of the antigen presenting cell, for a period of time sufficient to activate said T cell.
17 . The method of claim 16 , wherein the antigen presenting cell is infected with a recombinant virus expressing the peptide.
18 . The method of claim 11 , wherein the cancer is head and neck squamous cell carcinoma.
19 . The method of claim 11 , wherein the cancer is esophageal cancer.
20 . The method of claim 15 , wherein said adjuvant comprises IL-21.