Methods and compositions for nucleic acid analysis
Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
1. A method of capturing cells with specific cell surface markers and analyzing polynucleotides from the cells, the method comprising,
providing antibodies associated with a unique antibody-specific barcode sequence followed by;
coating cells with the antibodies such that the antibodies bind to target proteins on the cells to form antibody-coated cells followed by;
capturing (i) the antibody-coated cells and (ii) first droplets containing cell-specific barcode adaptors in second droplets followed by;
fusing contents of the first droplet with contents of the second droplet; and
lysing the cells in the second droplets followed by;
tagging polynucleotides in the second droplets with the cell-specific barcode adaptors to form tagged polynucleotides followed by;
sequencing the tagged polynucleotides, wherein adaptor sequences in sequencing reads can be used to infer which reads came from which cell.
2. The method of claim 1 , wherein the polynucleotides comprise DNA.
3. The method of claim 1 , wherein the polynucleotides comprise cDNA reverse transcribed from RNA.