Biochars, biochar extracts and biochar extracts having soluble signaling compounds and method for capturing material extracted from biochar
A method for capturing material extracted from biochar is provided comprising the steps of: (i) providing a biochar; (ii) contacting the biochar with an extraction media, where the extraction media causes the removal of residual compounds from the pores and surface of the biochar, creating a resulting extract comprised of the extraction media and removed compounds; and (iii) collecting the resulting extract. The method also can include other steps of extraction and purification. The method further comprises the step of applying the resulting extract to seeds, plants, soil, other agricultural products, or for use in other applications. A biochar having high levels of soluble signaling compounds is also provided, where the biochar is derived from a biomass source that together with predefined pyrolysis parameters produces resulting biochar having increased levels of soluble signaling compounds that are known to increase seed germination rates and early plant growth.
1. A method for capturing material extracted from biochar, the method comprising the steps of:
providing a biochar that includes soluble signaling compounds;
contacting the biochar with an extraction media, wherein the extraction media is a solvent other than water and removes at least some of the soluble signaling compounds from the biochar, thereby creating a biochar extract containing soluble signaling compounds;
collecting the biochar extract and further processing the biochar extract to remove the majority of biochar particles.
2. The method of claim 1 wherein the biochar extract is further processed to concentrate the soluble signaling compounds.
3. The method of claim 1 further including the step of applying the biochar extract to seeds, soil, or plants.
4. The method of claim 1 further including the step of reincorporating the biochar extract into a biochar.
5. The method of claim 1 where the soluble signaling compounds include one or more members selected from the group consisting of:
cyanohydrins, butenolides and karrikins.
6. The method of claim 1 where the starting biochar has karrikin levels that exceed 10 ng/g.
7. The method of claim 1 , where the extraction media includes one or more solvents selected from the group consisting of: acidic solutions, alkaline solutions, acetone, acetonitrile, ethyl acetate, organic acids, ethanol, polar solvents, non-polar solvents, ethers, chloroform, furans, ketones, phenolics, or dichloromethane.
8. A method for capturing material extracted from biochar, the method comprising the steps of:
providing a biochar that includes soluble signaling compounds;
contacting the biochar with an extraction media, wherein the extraction media is a solvent other than water and removes at least some of the soluble signaling compounds from the biochar, thereby creating a biochar extract containing soluble signaling compounds;
collecting the biochar extract and further processing the biochar extract to concentrate the soluble signaling compounds.
9. The method of claim 8 further including the step of applying the biochar extract to seeds, soil, or plants.
10. The method of claim 8 further including the step of reincorporating the biochar extract into a biochar.
11. The method of claim 8 where the soluble signaling compounds includes one or more members selected from the group consisting of:
cyanohydrins, butenolides and karrikins.
12. The method of claim 8 , where the extraction media includes one or more solvents selected from the group consisting of: acidic solutions, alkaline solutions, acetone, acetonitrile, ethyl acetate, organic acids, ethanol, polar solvents, non-polar solvents, ethers, chloroform, furans, ketones, phenolics, or dichloromethane.
13. A method for capturing material extracted from biochar, the method comprising the steps of:
providing a biochar that includes soluble signaling compounds;
contacting the biochar with an extraction media, wherein the extraction media is a solvent other than water and removes at least some of the soluble signaling compounds from the biochar, thereby creating a biochar extract containing soluble signaling compounds;
collecting the biochar extract; and
applying the biochar extract to seeds, soil, or plants.
14. The method of claim 13 where the soluble signaling compounds includes one or more members selected from the group consisting of:
cyanohydrins, butenolides and karrikins.
15. The method of claim 13 , where the extraction media includes one or more solvents selected from the group consisting of: acidic solutions, alkaline solutions, acetone, acetonitrile, ethyl acetate, organic acids, ethanol, polar solvents, non-polar solvents, ethers, chloroform, furans, ketones, phenolics, or dichloromethane.
16. A method for capturing material extracted from biochar, the method comprising the steps of:
providing a biochar that includes soluble signaling compounds;
contacting the biochar with an extraction media, wherein the extraction media removes at least some of the soluble signaling compounds from the biochar, thereby creating a biochar extract containing soluble signaling compounds;
collecting the biochar extract and reincorporating the biochar extract into a biochar.
17. The method of claim 16 where the soluble signaling compounds includes one or more members selected from the group consisting of:
cyanohydrins, butenolides and karrikins.
18. The method of claim 16 , where the extraction media is either or both water and ethanol.