Granted Patent
B2
US 12,290,533 · App. 16/972,372 · Granted May 6, 2025
Extracellular vesicles and methods of using
Assignee:
NUtech Ventures
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Abstract
The present disclosure relates to materials and methods for extracellular vesicle (e.g., exosome)-mediated delivery of cargo (e.g., endogenous and/or exogenous) to non-bovine mammalian (e.g., human) cells. For example, exosomes isolated from bovine milk for delivering cargo to non-bovine mammalian (e.g., human) cells are provided.
Claims (13)
1. A milk exosome comprising a biological membrane surrounding a lumen, wherein said milk exosome comprises an exogenous microRNA (miRNA) and an exogenous cargo encapsulated in said lumen, wherein said exogenous miRNA is biologically active in a mammal.
2. The milk exosome of claim 1 , wherein said milk exosome is isolated from sheep, goat, camel, horse, donkey, reindeer, yak, buffalo, or bovine (cow) milk or colostrum.
3. The milk exosome of claim 1 , wherein said miRNA is selected from the group consisting of MiR-320a, MiR-34a, miR-155-5p, and miR-375.
4. The milk exosome of claim 1 , wherein said exogenous cargo is selected from nucleic acid molecules, polypeptides, lipids, vitamins, minerals, small molecules, pharmaceuticals, hormones, enzymes, or any combination thereof.
5. The milk exosome of claim 1 , wherein the exogenous cargo comprises a therapeutic agent.
6. The milk exosome of claim 5 , wherein the therapeutic agent is selected from mRNAs, polypeptides, miRNAs, miRNA antagonists, nutrients, antibiotics, cancer drugs, activators of Toll-like receptors, or molecules capable of delivery to macrophages.
7. The milk exosome of claim 6 , wherein the therapeutic agent is a cancer drug selected from a chemotherapeutic, an immunotherapeutic, a hormone therapeutic, or a targeted therapeutic.
8. The milk exosome of claim 1 , wherein the exogenous cargo comprises a nutritional agent.
9. The milk exosome of claim 8 , wherein the nutritional agent is selected from vitamins, minerals, lipids, fatty acids, mRNAs, or polypeptides.
10. The milk exosome of claim 9 , wherein the nutritional agent is a fatty acid selected from omega-3 fatty acids or omega-6 fatty acids.
11. A composition formulated for oral administration to a human comprising the milk exosome of claim 1 .
12. A nutritional supplement or infant formula comprising the milk exosome of claim 1 .
13. The nutritional supplement or infant formula of claim 12 , wherein the milk exosome comprises a nutritional agent selected from vitamins, minerals, lipids, fatty acids, mRNAs, polypeptides, or any combination thereof.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 27, 2021
From: ZEMPLENI, JANOS
To: BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA
Reel/Frame 057307/0199 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Aug 27, 2021
From: BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA
To: NUTECH VENTURES
Reel/Frame 057308/0427 →
Continuity (3)
Provisional Application
62745951
· Oct 15, 2018
Provisional Application
62681592
· Jun 6, 2018
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