Nitric oxide releasing suppositories and methods of use thereof
The present invention relates generally to suppositories that release nitric oxide, and methods of using the same. According to some embodiments of the present invention provided herein is a nitric oxide (NO) releasing suppository (e.g., vaginal suppositories) are described herein. Some embodiments are directed to compositions, kits, and/or methods for treating and/or preventing an infection (e.g., a viral infection). In some embodiments, a method of treating and/or preventing a viral infection in a subject in need thereof is provided.
1. A suppository comprising:
a nitric oxide-releasing active pharmaceutical ingredient, wherein the nitric oxide-releasing active pharmaceutical ingredient is a macromolecule that comprises a crosslinked or non-crosslinked polymer, dendrimer, metallic compound, organometallic compound, or inorganic-based compound, wherein the macromolecule has a molecular weight of 500 Daltons or greater;
a suppository base; and
a proton source;
wherein the nitric oxide-releasing active pharmaceutical ingredient is within the suppository base, and
wherein the suppository releases nitric oxide in an amount of about 0.01% to about 10% by weight of the suppository, as measured by real time in vitro release testing.
2. The suppository of claim 1 , wherein the nitric oxide-releasing active pharmaceutical ingredient is present in an amount of about 0.1% to about 70% by weight of the suppository.
3. The suppository of claim 1 , wherein the suppository base is present in an amount of about 0.01% to about 99.91% by weight of the suppository.
4. The suppository of claim 1 , wherein the suppository base comprises theobroma oil, triglyceride, monoglyceride and diglyceride esters of C 8 -C 20 fatty acids and the mixtures thereof, gelatin, polyethylene glycol (PEG), and/or glycerolated glycerin, and
wherein the fatty acids comprise capric acid, caprylic acid, eicosenoic acid, stearic acid, lauric acid, myristic acid, oleic acid, palmitic acid, ricinoleic acid and their derivatives.
5. The suppository of claim 1 , wherein the suppository base is hydrophobic or hydrophilic.
6. The suppository of claim 1 , wherein the suppository further comprises a buffering agent in an amount of about 0.1% to about 30% by weight of the suppository.
7. The suppository of claim 1 , wherein the suppository further comprises at least two buffering agents, and
wherein each of the at least two buffering agents is present in an amount of about 0.1% to about 30% by weight of the suppository.
8. The suppository of claim 6 , wherein the buffering agent is selected from the group consisting of potassium phosphate monobasic, phosphoric acid, citric acid, acetic acid, lactic acid, boric acid, succinic acid, malic acid, sodium citrate dihydrate, and any combination thereof.
9. The suppository of claim 1 , wherein the suppository further comprises a preservative in an amount of about 0.1% to about 2% by weight of the suppository.
10. The suppository of claim 1 , wherein the suppository further comprises a solvent in an amount of about 0.1% to about 99% by weight of the suppository.
11. The suppository of claim 10 , wherein the solvent is selected from the group consisting of acetone, methyl alcohol, ethanol, isopropanol, butyl alcohol, ethyl acetate, dimethyl isosorbide, propylene glycol, glycerin, ethylene glycol, polyethylene glycol, diethylene glycol monoethyl ether, water, and any combination thereof.
12. The suppository of claim 1 , wherein the suppository further comprises a lubricant in an amount of about 1% to about 10% by weight of the suppository.
13. The suppository of claim 12 , wherein the lubricant is selected from the group consisting of magnesium stearate, calcium stearate, zinc stearate, hydrogenated vegetable oils, sterotex, polyoxyethylene monostearate, talc, polyethyleneglycol, sodium benzoate, sodium lauryl sulfate, magnesium lauryl sulfate, mineral oil, and any combination thereof.
14. The suppository of claim 1 , wherein the suppository further comprises mineral oil in an amount of about 1% to about 20% by weight of the suppository.
15. The suppository of claim 1 , wherein the suppository further comprises one or more hard waxes in an amount of about 1% to about 20% by weight of the suppository.
16. The suppository of claim 1 , wherein the nitric oxide-releasing active pharmaceutical ingredient comprises a co-condensed silica network comprising diazeniumdiolated methylaminopropyl trimethoxysilane (MAP3) and tetraethyl orthosilicate (TEOS).
17. The suppository of claim 1 , wherein the suppository administers nitric oxide in an amount sufficient to induce apoptosis in virally infected cells.
18. The suppository of claim 1 , wherein the suppository administers nitric oxide in an amount sufficient to reduce or eliminate viral replication with less than about 50% host cell cytotoxicity.
19. The suppository of claim 1 , wherein the suppository is a vaginal suppository.
20. The suppository of claim 1 , wherein the suppository is a single phase system.
21. A suppository comprising:
a nitric oxide-releasing active pharmaceutical ingredient, wherein the nitric oxide-releasing active pharmaceutical ingredient is a macromolecule that comprises a crosslinked or non-crosslinked polymer, dendrimer, metallic compound, organometallic compound, or inorganic-based compound, wherein the macromolecule has a molecular weight of 500 Daltons or greater;
a suppository base; and
a buffering agent in an amount of about 0.1% to about 30% by weight of the suppository;
wherein the nitric oxide-releasing active pharmaceutical ingredient is within the suppository base, and
wherein the suppository releases nitric oxide in an amount of about 0.01% to about 10% by weight of the suppository, as measured by real time in vitro release testing.
22. The suppository of claim 21 , wherein the buffering agent is selected from the group consisting of potassium phosphate monobasic, phosphoric acid, citric acid, acetic acid, lactic acid, boric acid, succinic acid, malic acid, sodium citrate dihydrate, and any combination thereof.
23. The suppository of claim 21 , wherein the nitric oxide-releasing active pharmaceutical ingredient comprises a co-condensed silica network comprising diazeniumdiolated methylaminopropyl trimethoxysilane (MAP3) and tetraethyl orthosilicate (TEOS).
24. The suppository of claim 21 , wherein the suppository is a vaginal suppository.