Brassica plant resistant to
The present invention relates to clubroot resistance in Brassica . More specifically, the invention relates to the CRT clubroot resistance gene, as well as to methods to create clubroot resistant Brassica plants.
1. A method for obtaining a clubroot resistant Brassica napus plant, comprising:
a) introducing or providing the clubroot resistance gene encoding a protein comprising an amino acid sequence of SEQ ID NO: 3, or an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 3, to a Brassica napus plant cell, to create a Brassica napus cell, and
b) regenerating a plant from said cell.
2. A method for obtaining a clubroot resistant Brassica napus plant, comprising:
crossing a first Brassica napus plant that comprises a nucleic acid molecule comprising a nucleotide sequence that encodes an amino acid sequence of SEO ID NO: 3, or an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 3, with a second Brassica plant that lacks the nucleic acid molecule to provide progeny Brassica napus plant; and
selecting a Brassica napus progeny plant that tests positive for the presence of the nucleotide sequence.
3. The Brassica napus plant obtained by the method according to claim 1 .
4. The Brassica napus plant according to claim 3 , further comprising at least one other disease resistance gene, said other disease resistance gene is a clubroot resistance gene, a blackleg resistance gene, a Sclerotinia resistance gene, a Verticillium resistance gene, a Fusarium resistance gene, an Aster Yellows resistance gene, an Alternaria resistance gene, and/or a Grey Stem resistance gene.
5. Seeds grown on the Brassica napus plant of claim 3 , wherein said seeds comprise a nucleic acid molecule comprising a nucleotide sequence that encodes an amino acid sequence of SEO ID NO: 3, or an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 3.
6. Seeds according to claim 5 which are hybrid seeds.
7. The hybrid seeds of claim 6 , which are Brassica napus hybrid seeds, and wherein said hybrid seeds develop into plants, the solid component of the seeds contains less than 30 micromoles of any one or any mixture of 3-butenyl glucosinolate, 4-pentenyl glucosinolate, 3-hydroxy-3 butenyl glucosinolate, and 2-hydroxy-4-pentenyl glucosinolate per gram of air-dry, oil-free solid.