Increased bioavailability of transdermally delivered agents
A method for delivering a bioactive agent to the cardiovascular system is described. The method delivers the agent at a high bioavailability and with little loss of agent to the natural defense mechanisms of the body. For instance, little or none of the bioactive agent will be sequestered in lymph tissue and prevented from circulation in the cardiovascular system. The method includes utilization of a transdermal delivery device including microneedles with structures fabricated on a surface of the microneedles to form a nanotopography. A random or non-random pattern of structures may be fabricated such as a complex pattern including structures of differing sizes and/or shapes.
1. A method of delivering a bioactive agent to a subject, the method comprising:
penetrating a stratum corneum of the subject with a microneedle having a channel in fluid communication with the bioactive agent, a plurality of nanostructures and a plurality of microstructures formed on an external surface of the microneedle, wherein at least a portion of the microstructures have a cross-sectional dimension of greater than about 500 nanometers and less than about 10 micrometers and a height of from about 20 nanometers to about 1 micrometer, wherein at least a portion of the microstructures have a cross-sectional dimension greater than a height of the microstructures, the plurality of nanostructures having a fractal and/or a fractal-like geometry;
transporting the bioactive agent through the channel of the microneedle; and
delivering the bioactive agent to the subject with a comparative bioavailability as compared to subcutaneous delivery of greater than about 20%;
wherein the stratum corneum comprises tight junctions between cells, the nanostructures rearranging tight junctions between the cells, thereby increasing porosity; and
wherein the rearrangement of the tight junctions leads to an increase in the porosity of a second tissue type that is not in direct contact with the microneedle.
2. The method according to claim 1 , wherein the bioactive agent is delivered to the subject such that the concentration of the bioactive agent in lymph node tissue of the subject is less than about 50 nanograms per gram of the lymph node tissue about 72 hours after penetrating the stratum corneum of the subject.
3. The method according to claim 2 , wherein the concentration of the bioactive agent in the lymph node is less than about 10 nanograms per gram of the lymph node about 72 hours after the application of the transdermal delivery device to the skin surface.
4. The method according to claim 3 , wherein the concentration of the bioactive agent in the lymph node is less than about 1 nanogram per gram of the lymph node about 72 hours after the application of the transdermal delivery device to the skin surface.
5. The method according to claim 1 , the subject having a spleen, wherein the concentration of the bioactive agent in a sample of the spleen tissue is less than about 5 nanograms per gram of the sample of the spleen tissue about 72 hours after the application of the transdermal delivery device to the skin surface.
6. The method according to claim 5 , wherein the concentration of the bioactive agent in a sample of the spleen tissue is less than about 1 nanogram per gram of the sample of the spleen tissue about 72 hours after the application of the transdermal delivery device to the skin surface.
7. The method according to claim 1 , the subject having a liver, wherein the concentration of the bioactive agent in a sample of the liver tissue is less than about 50 nanograms per gram of the sample of liver tissue about 72 hours after the application of the transdermal delivery device to the skin surface.
8. The method according to claim 7 , wherein the concentration of the bioactive agent in a sample of the liver tissue is less than about 1 nanogram per gram of the sample of the liver tissue about 72 hours after the application of the transdermal delivery device to the skin surface.
9. The method according to claim 1 , wherein the second tissue type is vascular tissue.
10. The method according to claim 1 , wherein the bioactive agent has a molecular weight of greater than about 100 kDa.
11. The method according to claim 1 , wherein the bioactive agent is selected from the group consisting of a protein therapeutic, an immunoglobulin, an antiviral agent, an anti-inflammatory agent and a vaccine.
12. The method according to claim 11 , wherein the anti-inflammatory agent is a disease-modifying antirheumatic drug.
13. A device for delivering a bioactive agent to a subject, the device comprising:
a microneedle having a channel in fluid communication with the bioactive agent, a plurality of nanostructures and a plurality of microstructures formed on an external surface of the microneedle, wherein at least a portion of the microstructures have a cross-sectional dimension of greater than about 500 nanometers and less than about 10 micrometers and a height of from about 20 nanometers to about 1 micrometer, wherein at least a portion of the microstructures have a cross-sectional dimension greater than a height of the microstructures, the plurality of nanostructures having a fractal and/or a fractal-like geometry; and
wherein the device is configured to deliver the bioactive agent to the subject with a comparative bioavailability as compared to subcutaneous delivery of greater than about 20%.
14. The device according to claim 13 , further comprising nanostructures having a cross-sectional dimension less than the cross-sectional dimension of the microstructures.
15. The device according to claim 13 , wherein at least a portion of the nanostructures of the plurality of nanostructures have a height from about 10 nanometers to about 1 micrometer.
16. The device according to claim 13 , wherein the geometry has a fractal dimension greater than about 1.
17. The device according to claim 13 , wherein the geometry has a fractal dimension between about 1.5 and about 2.5.
18. The device according to claim 13 , wherein the plurality of nanostructures has an average surface roughness between about 10 nanometers and about 200 nanometers.
19. The device according to claim 13 , wherein the plurality of nanostructures has an effective shear modulus between about 4 MPa and about 320 MPa.
20. The device according to claim 13 , wherein the channel has a cross-sectional dimension from about 1 micrometer to about 100 micrometers.
21. The device according to claim 20 , wherein the channel has a length from about 10 micrometers to about 800 micrometers.
22. The device according to claim 13 , wherein at least a portion of the nanostructures of the plurality of nanostructures have a cross-sectional dimension from about 20 nanometers to about 400 nanometers, and at least a portion of the microstructures of the plurality of microstructures have a cross-sectional dimension from about 600 nanometers to about 1.5 micrometers.
23. The device according to claim 13 , wherein each nanostructure of the plurality of nanostructures has approximately the same cross-sectional dimension.
24. The device according to claim 13 , wherein a ratio of the cross sectional dimension of two adjacent nanostructures of the plurality of nanostructures to the center-to-center spacing between those two nanostructures is between about 1:1 and about 1:4.
25. The device according to claim 13 , wherein at least a portion of the nanostructures of the plurality of nanostructures have an equidistant spacing.
26. The device according to claim 13 , where at least a portion of the nanostructures of the plurality of nanostructures have a height from about 20 nanometers to about 1 micrometer.
27. The device according to claim 13 , wherein at least a portion of the nanostructures of the plurality of nanostructures have an aspect ratio from about 0.2 to about 5.
28. The device according to claim 13 , wherein at least a portion of the nanostructures of the plurality of nanostructures have an aspect ratio from about 0.5 to about 3.5.
29. The device according to claim 13 , wherein at least a portion of the microstructures of the plurality of microstructures have an aspect ratio from about 0.15 to 1.
30. The device according to claim 13 , wherein at least a portion of the nanostructures of the plurality of nanostructures have a center-to-center spacing from about 50 nanometers to about 1 micrometer.
31. The device according to claim 13 , wherein each of the nanostructures of the plurality of nanostructures are in the form of pillars, the pillars having a base adjacent to the external surface of the microneedle and extending in a generally longitudinal direction to a tip.
32. The device according to claim 13 , wherein at least a portion of the nanostructures of the plurality of nanostructures have a height greater than a cross-sectional dimension.