HAIRPIN PRIMER DESIGN FOR SEQUENTIAL PCR PRODUCTION OF TARGETED SEQUENCING LIBRARIES
The present disclosure provides a method of hairpin primer design and targeted amplification thereof for creation of targeted sequencing libraries. Generally, the present methods allow for simultaneous construction of targeted sequencing libraries for multiple targeted nucleic acid sequences within a single sample. The presently disclosed methods generate efficient and specific target sequence amplification while avoiding, or significantly reducing, non-specific interaction of multiplex primers, non-specific amplification of sequences due to random priming from molecular “tags” (such as Molecular Identifiers (“MI”) barcodes), and unintentional interactions between gene-specific and universal primers.
1 . A method of library preparation, the method comprising:
partitioning a mixture comprising a nucleic acid, a hairpin primer, and a polymerase into a plurality of partitions, wherein the hairpin primer comprises a hairpin structure that inhibits non-specific interactions with the hairpin primer;
annealing, within one of the partitions, the hairpin primer to the nucleic acid; and
performing an amplification reaction to extend the annealed hairpin primer with the polymerase, thereby creating an amplicon.
2 . The method of claim 1 , further comprising performing a second amplification reaction with a universal primer that includes a targeting sequence complementary to a portion of the amplicon.
3 . The method of claim 2 , wherein the partitions are aqueous droplets surrounded by oil within a tube.
4 . The method of claim 3 , wherein the partitioning, the amplification reaction, and the second amplification reaction are performed within the tube and without lysing or releasing contents from the droplets.
5 . The method of claim 3 , wherein partitioning is achieved by vortexing the tube.
6 . The method of claim 3 , wherein the mixture further comprises a plurality of beads that template the formation of the droplets.
7 . The method of claim 2 , wherein the amplification reaction is performed at a first temperature and the second amplification reaction is performed at a second temperature lower than the first temperature and lower than a third temperature at which the hairpin structure denatures.
8 . The method of claim 7 , wherein the first temperature is in the range of about 50-70 degrees C. and the second temperature is in the range of about 55-80 degrees C.
9 . The method of claim 2 , wherein the universal primer further comprises one or more of an indexing sequence, a barcode sequence, and a sequencing adaptor.
10 . The method of claim 2 , wherein the hairpin primer comprises a molecular identifier sequence.
11 . The method of claim 10 , wherein the hairpin structure inhibits non-specific amplification of sequences by random priming via the molecular identifier sequence.
12 . The method of claim 1 , wherein the partitions comprise pipetted emulsions or microfluidically-generated droplets.
13 . The method of claim 1 , wherein the mixture further comprises a universal primer, the universal primer comprising a molecular identifier sequence and a priming sequence that is complementary to a portion of the amplicon.
14 . The method of claim 13 , wherein the hairpin structure of the hairpin primer prevents non-specific priming via the molecular identifier sequence.
15 . A composition for nucleic acid amplification, the composition comprising:
a plurality of aqueous partitions, wherein one of the partitions comprises:
a bead;
a hairpin primer comprising a stem and loop structure that inhibits non-specific hybridization;
a target nucleic acid; and
a polymerase.
16 . The composition of claim 15 , wherein the partitions comprise aqueous droplets formed and contained within a tube.
17 . The composition of claim 16 , wherein the droplets are formed by vortexing the tube.
18 . The composition of claim 17 , wherein the bead templates the formation of one of the droplets.
19 . The composition of claim 15 , wherein the one partition further comprises a universal primer that includes a molecular identifier sequence and priming sequence that is complementary to an amplicon created by extending the hairpin primer annealed to the target nucleic acid.
20 . The composition of claim 19 , wherein the stem and loop structure of the hairpin primer prevents non-specific priming via the molecular identifier sequence.