Tim protein-bound carrier, methods for obtaining, removing and detecting extracellular membrane vesicles and viruses using said carrier, and kit including said carrier
The invention provides a carrier and a method for obtaining, removing, or detecting extracellular membrane vesicle or virus present in a sample. In particular, the invention provides (a) a carrier (a Tim carrier) on which a protein (a Tim protein), selected from a T-cell immunoglobulin and mucin domain-containing molecule-4 (a Tim-4) protein, a Tim-3 protein, and a Tim-1 protein, is bound; (b) a method for obtaining the extracellular membrane vesicle or the virus in the sample; (c) a method for removing the extracellular membrane vesicle or the virus in the sample; (d) a method for detecting the extracellular membrane vesicle or the virus in the sample; (e) a kit for capturing the extracellular membrane vesicle or the virus, comprising the Tim carrier; and (f) a kit for capturing the extracellular membrane vesicle or the virus, comprising a reagent containing the Tim protein and a reagent containing the carrier.
1. A method for detecting an extracellular membrane vesicle in a sample, comprising the following steps:
(1) a step for forming a complex of Tim-4 protein bound to a carrier, and the extracellular membrane vesicle in the sample, in the presence of a calcium ion (complex formation step), and
(2) a step for detecting the complex using an anti extracellular membrane vesicle antibody (detection step).
2. The method according to claim 1 , wherein the method for detecting the extracellular membrane vesicle is an ELISA method or a flow cytometry method.
3. The method according to claim 1 , wherein the Tim-4 protein comprises an IgV domain.
4. The method according to claim 1 , wherein the carrier binds to the Tim-4 protein via an SH-group of the Tim-4 protein.