IMMUNOMODULATORY COMPOSITIONS AND METHODS OF USE THEREOF
Provided are immunomodulatory pharmaceutical and non-pharmaceutical compositions that include alpha-synuclein and at least one preselected antigen, such as at least one preselected peptide antigen or immunogen. Also provided are methods for modulating immune activity toward at least one preselected antigen in an at least substantially antigen-specific manner that include administering such a composition to a human patient or to a non-human mammalian subject. Still further provided are enhanced assay methods for quantifying antigen-specific cellular responses, such as cytokine release, to preselected antigens.
1 . In a cytokine release assay method comprising contacting T-cells ex vivo with at least one preselected antigen that is not alpha-synuclein and measuring the quantity of a cytokine released by the T-cells in response to said contacting, the improvement comprising contacting the T-cells with both the at least one preselected antigen and alpha-synuclein.
2 . The cytokine release assay method of claim 1 , wherein the method is an interferon-gamma release assay (IGRA) method and the cytokine is interferon-gamma.
3 . The cytokine release assay method of claim 1 , wherein the alpha-synuclein is human alpha-synuclein.
4 . The cytokine release assay method of claim 1 , wherein the alpha-synuclein is recombinant alpha-synuclein.
5 . The cytokine release assay method of claim 4 , wherein the recombinant alpha-synuclein is recombinant human alpha-synuclein.