Devices and methods for sample partitioning and analysis
The present disclosure provides a device that includes a base comprising a substrate having a first major surface, a pressure sensitive adhesive adhered to at least a portion of the first major surface, a polymeric cover film coupled to the substrate via the adhesive, a plurality of isolated closed compartments disposed between the substrate and the cover film, and an aqueous liquid disposed in two or more of the closed compartments. The cover film is a composite film comprising ethylene vinyl acetate copolymer, a linear copolymer of ethylene and a higher alkene, and a tackifier. Each compartment of the plurality is defined by a seal that prevents liquid communication with at least one other compartment of the plurality. Methods of using the device for partitioning a sample, for analyzing a sample, and for culturing a microorganism are also provided.
1. A method of incubating and analyzing a liquid comprising
depositing a liquid device onto a first surface of a substrate of a device such that the liquid sample is disposed between the first surface and a polymeric cover film wherein said first surface is coated with water-insoluble pressure sensitive adhesive;
wherein the polymeric cover film is a composite film comprising a polymer and a tackifier;
urging an external means against the cover film to bring discrete regions of the cover film in contact with the pressure sensitive adhesive of the substrate resulting in the partitioning of the liquid into a plurality of closed compartments disposed between the substrate and the cover film;
incubating the liquid under conditions that facilitate at least one cell division and
conducting a most probable number analysis for a microorganism in the device.
2. The method of claim 1 , wherein the most probable number analysis is conducted on the liquid within the plurality of closed compartments a single device.
3. The method of claim 1 , wherein the microorganisms is E. coli or S. aureus.
4. The method of claim 1 , wherein the plurality of closed compartments are microcompartments, each having a volume that is a microvolume.
5. The method of claim 1 , wherein the device comprises 100 to 300 closed compartments and the method comprises partitioning the liquid among in the 100 to 300 closed compartments.
6. The method of claim 1 , further comprising urging an external means against the cover film to bring regions of the cover film in contact with the pressure sensitive adhesive on the substrate.
7. The method of claim 1 , wherein the method does not comprise performing serial dilutions on the liquid.
8. The method of claim 1 , wherein the method does not include a step of cross-contaminating between the plurality of closed compartments.
9. The method of claim 1 , further comprising placing a base of the device in contact with a compliant surface while bringing regions of the cover film in contact with the pressure sensitive adhesive.
10. The method of claim 1 , wherein the step of bringing regions of the cover film in contact with the pressure sensitive adhesive seals the plurality of closed compartments comprises sealing the plurality of closed compartments.
11. The method of claim 9 , wherein the base of the device comprises a compliant member.
12. The method of claim 1 , wherein the plurality of closed compartments comprise a nutrient growth medium.
13. The method of claim 12 , wherein the nutrient growth medium is a selective growth medium.
14. The method of claim 12 , wherein the nutrient growth medium comprises at least one indicator substance.
15. The method of claim 1 , wherein the microorganism is an anaerobic microorganism and the device is kept in an anaerobic environment during the incubation step.
16. The method of claim 1 , wherein the step of incubating is conducted at 25° C. to 45° C.