Fluidic system for performing assays
A fluidic testing system and method for use are presented. The fluidic testing system includes a microfluidic channel, a first chamber and second chamber. The microfluidic channel has only one port for the introduction and/or extraction of fluid through the microfluidic channel. The first chamber is disposed at a terminal end of the microfluidic channel. The second chamber is coupled to the fluidic channel and is aligned such that each opening to the second chamber is configured to be aligned substantially parallel to a gravity vector during operation.
1. A method comprising:
flowing a liquid through an only fluid port of a fluidic channel in a first direction until the liquid reaches a first set of one or more reagents stored in a first chamber coupled to the fluidic channel, wherein the liquid flows through a second chamber coupled to the fluidic channel before reaching the first chamber;
re-suspending at least a portion of the first set of one or more reagents within the liquid to form a target liquid;
flowing the target liquid back through the fluidic channel to the second chamber in a second direction and away from the first chamber;
flowing the target liquid back and forth within the fluidic channel, such that the target liquid flows through the second chamber;
reacting at least a portion of the re-suspended first set of one or more reagents within the target liquid with a second set of one or more reagents disposed within the second chamber, the second set of one or more reagents being immobilized on a surface of a reagent insert plugged into a through-hole aligned with the second chamber, wherein the reacting begins once the re-suspended first set of one or more reagents contacts the second set of one or more reagents; and
flowing the target liquid out of the fluidic channel via the only fluid port of the fluidic channel.
2. The method of claim 1 , wherein each opening to the second chamber is aligned substantially parallel to a gravity vector.
3. The method of claim 1 , wherein flowing the target liquid back and forth within the fluidic channel comprises flowing the target liquid back and forth between two locations of the fluidic channel.
4. The method of claim 1 , further comprising:
sensing a presence of the liquid at each of the two locations of the fluidic channel.
5. The method of claim 1 , wherein flowing the target liquid back and forth within the fluidic channel comprises flowing the target liquid through a third chamber and mixing the target liquid as it flows through the third chamber.
6. The method of claim 1 , further comprising:
measuring an optical signal from the second chamber, wherein the optical signal is associated with a concentration of the re-suspended first set of one or more reagents within the second chamber.
7. The method of claim 1 , further comprising:
flowing a second liquid through the only fluid port of the fluidic channel;
flowing the second liquid back and forth within the fluidic channel, such that the second liquid flows through the second chamber;
flowing the second liquid out of the fluidic channel via the only fluid port of the fluidic channel.
8. The method of claim 7 , wherein the second liquid is a wash buffer.
9. The method of claim 1 , wherein the reacting comprises binding at least a portion of the re-suspended first set of one or more reagents within the target liquid with the second set of one or more reagents.
10. The method of claim 1 , wherein:
the second chamber is aligned with a through-hole of a housing of the fluidic channel; and
the method further comprises inserting the second set of one or more reagents into the second chamber via the through-hole.
11. The method of claim 10 , wherein the inserting comprises inserting a reagent insert into the through-hole such that the insert fits within the through-hole, wherein the reagent insert has the second set of one or more reagents on a side of the reagent insert.