Observation device
An observation device includes: an illumination unit that generates illumination light to be radiated onto cells floating in a culture fluid inside a culture vessel; a light receiving unit that receives observation light coming from the cells that have been irradiated with the illumination light, the observation light being imaged by an image-forming optical system; and a casing that has a transmissive section so as to transmit the illumination light and the observation light and that accommodates the illumination unit and the light receiving unit, wherein the casing has an elongated cylindrical form that is configured to be inserted into the culture fluid via a port used to insert a tube into the culture vessel.
1. An observation device for observing cells floating in a culture fluid inside a culture vessel, the observation device comprising:
an illumination unit comprising a light source configured to generate illumination light to be radiated onto the cells;
a light receiving unit comprising an image sensor configured to receive observation light coming from the cells that have been irradiated with the illumination light, the observation light being imaged by an image-forming optical system; and
a casing that has a transmissive section so as to transmit the illumination light and the observation light and that accommodates at least a portion of the illumination unit and at least a portion of the light receiving unit,
wherein the casing has an elongated cylindrical form that is configured to be inserted into the culture fluid via a port used to insert a tube into the culture vessel.
2. The observation device according to claim 1 , wherein the illumination unit comprises a fiber that guides light emitted by the light source and that makes the light output from a distal end of the casing, the light source being disposed inside the casing side or being disposed outside the casing.
3. The observation device according to claim 1 , wherein the light receiving unit comprises a fiber, and
the fiber is configured to guide the received observation light to the image sensor disposed inside the casing or disposed outside the casing.
4. The observation device according to claim 1 , wherein the casing includes, at a position closer to a proximal end than a distal end thereof in a longitudinal direction is, a recessed section that is recessed radially inward, and
the transmissive section is provided on each of both side surfaces of the recessed section that are opposed to each other in the longitudinal direction.
5. The observation device according to claim 1 , wherein the casing is divided into a first casing that accommodates at least the portion of the illumination unit and a second casing that accommodates at least the portion of the light receiving unit,
the first casing and the second casing are disposed at a distance from each other in an optical-axis direction, and
the transmissive section is provided on each of surfaces of the first casing and the second casing, the surfaces being opposed to each other.
6. The observation device according to claim 1 , further comprising a cylindrical protective tube that covers around the casing,
wherein the protective tube has a transparent section, which is optically transparent, and has such an elongated shape as to be insertable into the culture fluid via the port.
7. The observation device according to claim 6 , wherein the protective tube is made of a transparent resin material.
8. The observation device according to claim 6 , wherein the protective tube has a protruding part that protrudes outward from the transparent section, and
the protruding part transmits and/or reflects, toward the cells, at least part of the illumination light that has been transmitted through the transparent section.
9. The observation device according to claim 1 , wherein the image-forming optical system is configured to provide at least one of a predetermined magnification, a predetermined depth of field, and a predetermined direction of the observation field of view.
10. The observation device according to claim 9 , wherein the image-forming optical system is disposed so as to face a side surface of the casing, and
the image-forming optical system includes a prism that reflects the observation light entering the image-forming optical system through the side surface of the casing toward the light receiving unit.
11. The observation device according to claim 1 , further comprising a processor comprising hardware, the processor being configured to count the number of the cells in a cell image generated on the basis of the observation light that has been received by the light receiving unit.
12. The observation device according to claim 11 , wherein the processor is configured to estimate the number and/or the density of the cells in the culture fluid by using two or more cell images that have been generated on the basis of the observation light received by the light receiving unit at times different from each other.
13. The observation device according to claim 12 , wherein the two or more cell images are generated on the basis of the observation light received by the light receiving unit at time intervals each longer than a duration of time from appearance of the same cell to disappearance thereof in each of the cell images.