Enterococcus gallinarum flagellin polypeptides
The invention provides compositions comprising flagellin polypeptides from bacterial strains and the use of such compositions in the treatment of disease.
1. A pharmaceutical composition that comprises an isolated Enterococcus flagellin polypeptide in a unit dose comprising the Enterococcus flagellin polypeptide and a pharmaceutically acceptable excipient, diluent, or carrier, wherein the pharmaceutical composition is an enteric formulation, and wherein the Enterococcus flagellin polypeptide is from an Enterococcus gallinarum strain and comprises an amino acid sequence with at least 98% sequence identity to SEQ ID NO: 1.
2. The pharmaceutical composition of claim 1 , wherein the composition comprises a therapeutically effective amount of the flagellin polypeptide sufficient to stimulate an immune response in a subject when administered as a unit dose to the subject.
3. The pharmaceutical composition of claim 1 , wherein the Enterococcus flagellin polypeptide comprises a polypeptide sequence with at least 99% sequence identity to the polypeptide sequence of SEQ ID NO: 1.
4. The pharmaceutical composition of claim 1 , wherein the Enterococcus flagellin polypeptide comprises a polypeptide sequence with 100% sequence identity to the polypeptide sequence of SEQ ID NO: 1.
5. The pharmaceutical composition of claim 1 , wherein the Enterococcus flagellin polypeptide is from the Enterococcus gallinarum strain deposited under accession number NCIMB 42488.
6. A pharmaceutical composition that comprises an isolated recombinant Enterococcus flagellin polypeptide in a unit dose comprising a therapeutically effective amount of the Enterococcus flagellin polypeptide to stimulate an immune response in a subject when administered to the subject and a pharmaceutically acceptable excipient, diluent, or carrier, wherein the pharmaceutical composition is an enteric formulation, and wherein the Enterococcus flagellin polypeptide comprises an amino acid sequence with at least 98% sequence identity to SEQ ID NO: 1.
7. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is a solid composition.
8. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is formulated as a tablet or a capsule.
9. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition is formulated for delivery to an intestine of the subject.
10. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition comprises a preservative, an antioxidant, or a stabilizer.
11. The pharmaceutical composition of claim 1 , wherein the at least 98% sequence identity to SEQ ID NO:1 is determined by a Smith-Waterman homology search algorithm using an affine gap search with a gap open penalty of 12 and a gap extension penalty of 2.