DETECTION OF COLORECTAL NEOPLASMS
The present disclosure provides, among other things, methods for colorectal neoplasm detection (e.g., screening) and compositions related thereto. In various embodiments, the present disclosure provides methods for adenoma and/or early stage colorectal cancer detection (e.g., screening) and compositions related thereto. In various embodiments, the present disclosure provides methods for screening that include analysis of methylation status of one or more methylation biomarkers, and compositions related thereto. In various embodiments, the present disclosure provides methods for detection (e.g., screening) that include detecting (e.g., screening) methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA. In various embodiments, the present disclosure provides methods for screening that include detecting (e.g., screening) methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA, using MSRE-qPCR and/or using massively parallel sequencing (e.g., next-generation sequencing).
1 . A method of detecting a colorectal neoplasm in a human subject, the method comprising:
determining a methylation status of each of one or more markers identified in a sample obtained from the subject, and
determining whether the subject has a colorectal neoplasm based at least in part on the determined methylation status of each of the one or more markers,
wherein each of the one or more markers is a methylation locus comprising at least a portion of a differentially methylated region (DMR) selected from the DMRs of Table 1.
2 . The method of claim 1 , wherein detecting the colorectal neoplasm comprises a member selected from the group consisting of (i) classifying the subject as having advanced adenoma, (ii) classifying the subject as having polyposis, (iii) classifying the subject as having colorectal cancer, (iv) classifying the subject as having at least one of the conditions advanced adenoma, polyposis, and colorectal cancer, either with or without identifying which of those conditions the subject has, and (v) classifying the subject as having at least one of the conditions advanced adenoma and colorectal cancer, either with or without identifying which of those conditions the subject has.
3 . The method of claim 1 , wherein the sample is or comprises a blood sample, a blood product sample, a stool sample, a colorectal tissue sample.
4 . The method of claim 1 , wherein the method comprises determining a methylation status of at least a portion of each of one or more of the following DMRs: SLC6A1 ′689 [chr3:10993689-10993900] (SEQ ID NO: 9); F13A1 ′205 [chr6:6320205-6320663] (SEQ ID NO: 31); BARHL1 ′614 [chr9:132579614-132579683] (SEQ ID NO: 41); and [chr19:20052466-20053193] (SEQ ID NO: 53).
5 . The method of claim 1 , wherein the method comprises determining a methylation status of a portion of each of one or more of the following DMRs: CSMD2 ′443 [chr1:34165443-34165675] (SEQ ID NO:1); SLC6A1 ′689 [chr3:10993689-10993900] (SEQ ID NO: 9); [chr6:27670532-27670614] (SEQ ID NO: 33); and [chr19:20052466-20053193] (SEQ ID NO: 53).
6 . The method of claim 1 , wherein the method comprises determining the methylation status of each of the one or more markers using next generation sequencing (NGS).
7 . The method of claim 6 , comprising using one or more oligonucleotide capture baits that enrich for a target region to capture one or more corresponding methylation locus/loci.
8 . The method of claim 1 , wherein the one or more marker(s) is or comprises at least one CpG dinucleotide.
9 . The method of claim 1 , wherein the step of determining the methylation status further comprises determining a relative amount of methylated and unmethylated CpGs and/or determining a read-based pathological methylation pattern.
10 . The method of claim 1 , wherein methylation status is determined using methylation sensitive restriction enzyme quantitative polymerase chain reaction (MSRE-qPCR).
11 . A method of detecting a colorectal neoplasm in a human subject, the method comprising:
determining a methylation status for each of one or more of the following, in deoxyribonucleic acid (DNA) of a human subject:
(i) a methylation locus within gene SLC6A1;
(ii) a methylation locus within gene F13A1; and
(iii) a methylation locus within gene BARHL1; and
diagnosing colorectal neoplasm in the human subject based at least on said determined methylation status(es).
12 . The method of claim 11 , wherein detecting the colorectal neoplasm comprises a member selected from the group consisting of (i) classifying the subject as having advanced adenoma, (ii) classifying the subject as having polyposis, (iii) classifying the subject as having colorectal cancer, (iv) classifying the subject as having at least one of the conditions advanced adenoma, polyposis, and colorectal cancer, either with or without identifying which of those conditions the subject has, and (v) classifying the subject as having at least one of the conditions advanced adenoma and colorectal cancer, either with or without identifying which of those conditions the subject has.
13 . The method of claim 11 , comprising determining a methylation status for a methylation locus within gene SLC6A1, wherein the methylation locus within gene SLC6A1 comprises at least a portion of SLC6A1 ′689 [chr3:10993689-10993900] (SEQ ID NO: 9)
14 . The method of claim 11 , comprising determining a methylation status for a methylation locus within gene F13A1, wherein the methylation locus within gene F13A1 comprises at least a portion of F13A1 ′205 [chr6:6320205-6320663] (SEQ ID NO: 31).
15 . The method of claim 11 , comprising determining a methylation status for a methylation locus within gene BARHL1, wherein the methylation locus within gene BARHL1 comprises at least a portion of BARHL1 ′614 [chr9:132579614-132579683] (SEQ ID NO: 41).
16 . The method of claim 11 , further comprising determining a methylation status for a methylation locus comprising at least a portion of chr19:20052466-20053193 in deoxyribonucleic acid (DNA) of the human subject, and wherein the diagnosing step comprises diagnosing colorectal neoplasm in the human subject based at least on the determined methylation status for the methylation locus comprising said at least portion of chr19:20052466-20053193 (SEQ ID NO: 53).
17 . The method of claim 11 , wherein the DNA is isolated from blood or plasma of the human subject.
18 . The method of claim 11 , wherein the step of determining the methylation status further comprises determining a relative amount of methylated and unmethylated CpGs and/or determining a read-based pathological methylation pattern.
19 . The method of claim 11 , wherein methylation status is determined using methylation sensitive restriction enzyme quantitative polymerase chain reaction (MSRE-qPCR).
20 . The method of claim 11 , comprising determining a methylation status for a methylation locus within gene SLC6A1, wherein the methylation locus of SLC6A1 is or comprises at least one CpG dinucleotide.
21 . The method of claim 11 , comprising determining a methylation status for a methylation locus within gene F13A1, comprising determining a methylation status for a methylation locus within gene F13A1, wherein the methylation locus of F13A1 is or comprises at least one CpG dinucleotide.
22 . The method of claim 11 , comprising determining a methylation status for a methylation locus within gene BARHL1, wherein the methylation locus of BARHL1 is or comprises at least one CpG dinucleotide.
23 . A method of detecting a colorectal neoplasm in a human subject, the method comprising:
determining a methylation status for each of one or both of the following, in deoxyribonucleic acid (DNA) of a human subject:
(i) a methylation locus within gene CSMD2; and
(ii) a methylation locus within gene SLC6A1; and
diagnosing the colorectal neoplasm in the human subject based at least on said determined methylation status(es).
24 . The method of claim 23 , wherein detecting the colorectal neoplasm comprises a member selected from the group consisting of (i) classifying the subject as having advanced adenoma, (ii) classifying the subject as having polyposis, (iii) classifying the subject as having colorectal cancer, (iv) classifying the subject as having at least one of the conditions advanced adenoma, polyposis, and colorectal cancer, either with or without identifying which of those conditions the subject has, and (v) classifying the subject as having at least one of the conditions advanced adenoma and colorectal cancer, either with or without identifying which of those conditions the subject has.
25 . The method of claim 23 , comprising determining a methylation status for a methylation locus within gene CSMD2, wherein the methylation locus of gene CSMD2 comprises at least one CpG dinucleotide.
26 . The method of claim 23 , comprising determining a methylation status for a methylation locus within gene SLC6A1, wherein the methylation locus of gene SLC6A1 comprises at least one CpG dinucleotide.
27 . The method of claim 23 , comprising determining a methylation status for a methylation locus within gene CSMD2, wherein the methylation locus within gene CSMD2 comprises at least a portion of CSMD2 ′443 [chr1:34165443-34165675] (SEQ ID NO: 1).
28 . The method of claim 23 , comprising determining a methylation status for a methylation locus within gene SLCA1, wherein the methylation locus within gene SLC6A1 comprises at least a portion of SLC6A1 ′689 [chr3:10993689-10993900] (SEQ ID NO: 9).
29 . The method of claim 23 , further comprising determining a methylation status for a methylation locus comprising at least a portion of chr6:27670532-27670614 (SEQ ID NO: 33) in deoxyribonucleic acid (DNA) of the human subject, and wherein the diagnosing step comprises diagnosing colorectal neoplasm in the human subject based at least on the determined methylation status for the methylation locus comprising said at least portion of chr6:27670532-27670614 (SEQ ID NO: 33).
30 . The method of claim 23 , further comprising determining a methylation status for a methylation locus comprising at least a portion of chr19:20052466-20053193 (SEQ ID NO: 53) in deoxyribonucleic acid (DNA) of the human subject, and wherein the diagnosing step comprises diagnosing colorectal neoplasm in the human subject based at least on the determined methylation status for the methylation locus comprising said at least portion of chr19:20052466-20053193 (SEQ ID NO: 53).
31 . The method of claim 23 , wherein the DNA is isolated from blood or plasma of the human subject.
32 . The method of claim 23 , wherein the DNA is cell-free DNA of the human subject.
33 . The method of claim 23 , wherein methylation status is determined using quantitative polymerase chain reaction (qPCR).
34 . The method of claim 23 , wherein methylation status is determined using methylation sensitive restriction enzyme quantitative polymerase chain reaction (MSRE-qPCR).
35 . The method of claim 23 , wherein methylation status is determined using massively parallel sequencing.
36 . The method of claim 23 , wherein each methylation locus is equal to or less than 5000 bp in length.
37 . The method of claim 23 , comprising determining the methylation status of each of the one or more markers using next generation sequencing (NGS).
38 . The method of claim 37 , comprising using one or more oligonucleotide capture baits that enrich for a target region to capture one or more corresponding methylation locus/loci.
39 . The method of claim 23 , wherein determining the methylation status further comprises determining a relative amount of methylated and unmethylated CpGs and/or determining a read-based pathological methylation pattern.
40 . A kit for use in a method detecting a colorectal neoplasm in a human subject, the kit comprising one or more oligonucleotide primer pairs for amplification of one or more corresponding methylation locus/loci of Table 1.
41 . A diagnostic qPCR reaction for detection of colorectal cancer, the diagnostic qPCR reaction including:
(i) human DNA,
(ii) a polymerase; and
(iii) one or more oligonucleotide primer pairs for amplification of one or more corresponding methylation locus/loci of Table 1, and, optionally, at least one methylation sensitive restriction enzyme.
42 . The diagnostic qPCR reaction of claim 41 , wherein each of the one or more corresponding methylation locus/loci comprise at least one methylation sensitive restriction enzyme (MSRE) cleavage site.
43 . A kit for use in a method of detecting a colorectal neoplasm in a human subject, the kit comprising one or more oligonucleotide capture baits for capturing one or more corresponding methylation locus/loci of Table 1.
44 . The kit of claim 43 , wherein the one or more oligonucleotide capture baits comprise one or more biotinylated oligonucleotide probes.