MDCK suspension cell lines in serum-free, chemically-defined media for vaccine production
Disclosed is an adapted Madin-Darby canine kidney cell line capable of suspension culture in the absence of serum, and a chemically-defined medium for culture of the adapted MDCK cell line. Further disclosed are culture methods for growing the adapted MDCK cell line and methods for producing a vaccine from the adapted MDCK cell line grown in the chemically-defined medium.
1. A cell culture comprising isolated adapted Madin-Darby canine kidney (MDCK) cells deposited as DSM ACC3309 with Leibniz-Institut DSMZ-Deutsche Sammlung von Mikro-organismen and Zellkulturen GmbH and a growth medium that comprises from about 20 mM to about 30 mM glucose.
2. The cell culture of claim 1 , wherein the growth medium is a chemically-defined and animal component free medium.
3. A method of producing an influenza virus for vaccine production, comprising contacting isolated adapted Madin-Darby canine kidney (MDCK) cells deposited as DSM ACC3309 with Leibniz-Institut DSMZ-Deutsche Sammlung von Mikro-organismen and Zellkulturen GmbH with a growth medium, infecting the isolated adapted MDCK cells with an influenza virus, and harvesting the influenza virus.
4. The method of claim 3 , wherein the growth medium is a chemically-defined and animal-component-free medium.
5. The method of claim 3 , wherein the growth medium comprises from about 20 mM to about 30 mM glucose.
6. The method of claim 3 , further comprising preparing a vaccine from the harvested influenza virus.
7. The method of claim 6 , wherein the vaccine is a human vaccine.
8. The method of claim 6 , wherein the prepared vaccine comprises a plurality of the harvested influenza viruses.
9. The method of claim 8 , wherein the plurality of harvested influenza viruses maintain antigenicity.