Optical reaction well for assay device
This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug having optically transmissive properties. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways.
1. A plug comprising:
A body with a bottom surface;
A cavity in the bottom surface;
A central opening in the body; and
A dried reagent on the bottom surface, wherein the dried reagent on the bottom surface is within the cavity, and wherein the body is formed from a material transmissive to excitation wavelengths and emission wavelengths in at least one of a red spectrum, a blue spectrum and a green spectrum.
2. The plug of claim 1 wherein a width of the central opening is wider than a portion of the bottom surface containing the dried reagent.
3. The plug of claim 1 further comprising a plug thickness between a central opening bottom and the plug body bottom wherein a depth of the cavity is less than 90% of the plug thickness.
4. The plug of claim 1 further comprising a plug thickness between a central opening bottom and the plug body bottom wherein a depth of the cavity is less than 70% of the plug thickness.
5. The plug of claim 1 further comprising a plug thickness between a central opening bottom and the plug body bottom wherein a depth of the cavity is less than 50% of the plug thickness.
6. The plug of claim 1 further comprising an annulus on the plug bottom surface from an outer edge of the plug body to a perimeter of the cavity.
7. The plug of claim 6 wherein the annulus completely encircles the perimeter of the cavity.
8. The plug of claim 1 the cavity further comprising a perimeter in the bottom surface wherein an angle of initiation of the cavity is measured from the perimeter relative to the bottom surface and the angle of initiation is 60 degrees or less.
9. The plug of claim 1 wherein the plug body central opening is wider than the cavity.
10. The plug of claim 1 the plug body bottom surface further comprising a bounded area on the plug body bottom surface wherein the dried reagent is within the bounded area.
11. The plug of claim 10 wherein the bounded area on the plug bottom surface is provided by a feature on the plug body bottom surface.
12. The plug of claim 11 wherein the feature is raised above the plug bottom surface or recessed into the plug bottom surface.
13. The plug of the claim 11 wherein the feature has a curved cross section or a rectangular cross section.
14. The plug of claim 10 wherein a width of the bounded area is less than a width of the body central opening, or a width of the bounded area is about the same as a width of the body central opening.
15. The plug of claim 1 wherein the plug has a polished or smooth finish facilitating the transmissivity of the excitation wavelengths and the emission wavelengths.
16. The plug of claim 1 further comprising a flange on the plug body around the central opening in the plug body.
17. The plug of claim 1 wherein the dried reagent is selected from the group consisting of nucleic acid synthesis reagents, peptide synthesis reagents, polymer synthesis reagents, nucleic acids, nucleotides, nucleobases, nucleosides, peptides, amino acids, monomers, detection reagents, catalysts or combinations thereof.
18. The plug of claim 1 wherein the dried reagent is a continuous film adhering to the plug bottom surface.
19. The plug of claim 1 wherein the dried reagent is a lyophilized reagent.
20. The plug of claim 1 wherein the dried reagent comprises one or more droplets adhering to the plug bottom surface.