Method for promoting proliferation of immune cells
Disclosed is a method for promoting immune cell proliferation. The method comprises the following step: upregulating the expression of low-density lipoprotein receptor-related proteins or fragments thereof in immune cells.
1. A genetically modified human immune cell, wherein the genetic modification up-regulates an expression of a low density lipoprotein receptor-associated protein or fragment thereof in the genetically modified immune cell, wherein the low density lipoprotein receptor-associated protein is low density lipoprotein receptor-associated protein 6 or the low density lipoprotein receptor-associated protein 5, wherein the low density lipoprotein receptor-associated protein or fragment thereof comprises an intracellular region of the low density lipoprotein receptor-associated protein, wherein the low density lipoprotein receptor-associated protein or fragment thereof comprises an amino acid sequence as set forth in any one of SEQ ID NO: 22, 24, 26, and 28, or an amino acid sequence having at least 80% homology thereof.
2. The genetically modified immune cell according to claim 1 , which expresses a chimeric antigen receptor (CAR) or a T cell receptor (TCR).
3. The genetically modified immune cell according to claim 1 , wherein the low density lipoprotein receptor-associated protein or fragment thereof is derived from a human.
4. The genetically modified immune cell according to claim 1 , wherein the low density lipoprotein receptor-associated protein 6 or fragment thereof further comprises an transmembrane region of the low density lipoprotein receptor-associated protein 6.
5. The genetically modified immune cell according to claim 1 , wherein the low density lipoprotein receptor-associated protein 5 or fragment thereof further comprises an transmembrane region of the low density lipoprotein receptor-associated protein 5.
6. A genetically modified human immune cell, wherein the genetic modification up-regulates an expression of a low density lipoprotein receptor-associated protein or fragment thereof in the genetically modified immune cell, wherein the low density lipoprotein receptor-associated protein is low density lipoprotein receptor-associated protein 6 or the low density lipoprotein receptor-associated protein 5, wherein the low density lipoprotein receptor-associated protein or fragment thereof comprises an intracellular region of the low density lipoprotein receptor-associated protein, wherein a nucleic acid molecule encoding the low density lipoprotein receptor-associated protein or fragment thereof comprises a nucleic acid sequence as set forth in any one of SEQ ID NO: 21, 23, 25, and 27.
7. A method for preparing the genetically modified immune cell of claim 1 , comprising: up-regulating an expression of the low density lipoprotein receptor-associated protein or fragment thereof in the genetically modified immune cell.
8. The method according to claim 7 , further comprising: introducing into the genetically modified immune cell a vector up-regulating the expression of the low density lipoprotein receptor-associated protein or fragment thereof.
9. The method according to claim 8 , wherein the vector is at least one selected from the group consisting of a retroviral vector, a lentiviral vector, and a transposon plasmid.