IP Library › Granted Patent US 12,133,542

Granted Patent B2

US 12,133,542 · App. 17/047,771 · Granted Nov 5, 2024

Animal feed compositions and uses thereof

Inventors: Rual Lopez-Ulibarri (Kaiseraugst, CH); Estefania Perez Calvo (Kaiseraugst, CH); Wolfgang Schliffka (Kaiseraugst, CH)

Assignees: Novozymes A/S; DSM IP Assets B.V.

A23K20/147 A23K10/18 A23K10/30 A23K20/174 A23K20/189 A23K50/30 A23K50/75 C12N9/2462 C12Y302/01017

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Quick Facts

Patent No.: US 12,133,542
App. No.: 17/047,771
Granted: Nov 5, 2024
Kind: B2

External Links

USPTO Patent Center↗ Full Patent PDF↗

Abstract

The present invention relates to animal feed compositions comprising at least one probiotic and polypeptides having muramidase activity.

Claims (395)

1. An animal feed or animal feed additive comprising a probiotic and a polypeptide having GH24 muramidase activity and/or GH25 muramidase activity, said probiotic comprising one or more Bacillus , one or more Lactobacillus , and/or one or more Clostridium.

2. The animal feed or animal feed additive of claim 1 , wherein the polypeptide is a fungal GH24 muramidase or GH25 muramidase.

3. The animal feed or animal feed additive of claim 1 , wherein the probiotic comprises one or more Lactobacillus , and/or one or more Bacillus.

4. The animal feed or animal feed additive of claim 1 , wherein the polypeptide having GH24 muramidase activity and/or GH25 muramidase activity degrades cell wall debris from Lactobacillus johnsonii.

5. The animal feed or animal feed additive of claim 1 , wherein the polypeptide having GH24 muramidase activity and/or GH25 muramidase activity is selected from the group consisting of:

(a) a polypeptide having at least 80% sequence identity to SEQ ID NO: 1;

(b) a polypeptide having at least 80% sequence identity to SEQ ID NO: 2;

(d) a polypeptide having at least 80% sequence identity to SEQ ID NO: 4;

(e) a polypeptide having at least 80% sequence identity to SEQ ID NO: 5;

(f) a polypeptide having at least 80% sequence identity to SEQ ID NO: 6;

(g) a polypeptide having at least 80% sequence identity to SEQ ID NO: 7;

(h) a polypeptide having at least 80% sequence identity to SEQ ID NO: 8;

(i) a polypeptide having at least 80% sequence identity to SEQ ID NO: 9;

(j) a polypeptide having at least 80% sequence identity to SEQ ID NO: 10;

(k) a polypeptide having at least 80% sequence identity to SEQ ID NO: 11;

(l) a polypeptide having at least 80% sequence identity to SEQ ID NO: 12;

(m) a polypeptide having at least 80% sequence identity to SEQ ID NO: 13;

(n) a polypeptide having at least 80% sequence identity to SEQ ID NO: 14;

(o) a polypeptide having at least 80% sequence identity to SEQ ID NO: 15;

(p) a polypeptide having at least 80% sequence identity to SEQ ID NO: 16;

(q) a polypeptide having at least 80% sequence identity to SEQ ID NO: 17;

(r) a polypeptide having at least 80% sequence identity to SEQ ID NO: 18;

(s) a polypeptide having at least 80% sequence identity to SEQ ID NO: 19;

(t) a polypeptide having at least 80% sequence identity to SEQ ID NO: 20;

(u) a polypeptide having at least 80% sequence identity to SEQ ID NO: 21;

(v) a polypeptide having at least 80% sequence identity to SEQ ID NO: 22;

(w) a polypeptide having at least 80% sequence identity to SEQ ID NO: 23;

(x) a polypeptide having at least 80% sequence identity to SEQ ID NO: 24;

(y) a polypeptide having at least 80% sequence identity to SEQ ID NO: 25;

(z) a polypeptide having at least 80% sequence identity to SEQ ID NO: 26;

(aa) a polypeptide having at least 80% sequence identity to SEQ ID NO: 27;

(ab) a polypeptide having at least 80% sequence identity to SEQ ID NO: 28;

(ac) a polypeptide having at least 80% sequence identity to SEQ ID NO: 29;

(ad) a polypeptide having at least 80% sequence identity to SEQ ID NO: 30;

(ae) a polypeptide having at least 80% sequence identity to SEQ ID NO: 31;

(af) a polypeptide having at least 80% sequence identity to SEQ ID NO: 32;

(ag) a polypeptide having at least 80% sequence identity to SEQ ID NO: 33;

(ah) a polypeptide having at least 80% sequence identity to SEQ ID NO: 34;

(ai) a polypeptide having at least 80% sequence identity to SEQ ID NO: 35;

(aj) a polypeptide having at least 80% sequence identity to SEQ ID NO: 36;

(ak) a polypeptide having at least 80% sequence identity to SEQ ID NO: 37;

(al) a polypeptide having at least 80% sequence identity to SEQ ID NO: 38;

(am) a polypeptide having at least 80% sequence identity to SEQ ID NO: 39;

(an) a polypeptide having at least 80% sequence identity to SEQ ID NO: 40;

(ao) a polypeptide having at least 80% sequence identity to SEQ ID NO: 41;

(ap) a polypeptide having at least 80% sequence identity to SEQ ID NO: 42;

(aq) a polypeptide having at least 80% sequence identity to SEQ ID NO: 43;

(ar) a polypeptide having at least 80% sequence identity to SEQ ID NO: 44;

(as) a polypeptide having at least 80% sequence identity to SEQ ID NO: 45;

(at) a polypeptide having at least 80% sequence identity to SEQ ID NO: 46;

(au) a polypeptide having at least 80% sequence identity to SEQ ID NO: 47;

(av) a polypeptide having at least 80% sequence identity to SEQ ID NO: 48;

(aw) a polypeptide having at least 80% sequence identity to SEQ ID NO: 49;

(ax) a polypeptide having at least 80% sequence identity to SEQ ID NO: 50;

(ay) a polypeptide having at least 80% sequence identity to SEQ ID NO: 51;

(az) a polypeptide having at least 80% sequence identity to SEQ ID NO: 52;

(ba) a polypeptide having at least 80% sequence identity to SEQ ID NO: 53;

(bb) a polypeptide having at least 80% sequence identity to SEQ ID NO: 54;

(bc) a polypeptide having at least 80% sequence identity to SEQ ID NO: 55;

(bd) a polypeptide having at least 80% sequence identity to SEQ ID NO: 56;

(be) a polypeptide having at least 80% sequence identity to SEQ ID NO: 57;

(bf) a polypeptide having at least 80% sequence identity to SEQ ID NO: 58;

(bg) a polypeptide having at least 80% sequence identity to SEQ ID NO: 59;

(bh) a polypeptide having at least 80% sequence identity to SEQ ID NO: 60;

(bi) a polypeptide having at least 80% sequence identity to SEQ ID NO: 61;

(bj) a polypeptide having at least 80% sequence identity to SEQ ID NO: 62;

(bk) a polypeptide having at least 80% sequence identity to SEQ ID NO: 63;

(bl) a polypeptide having at least 80% sequence identity to SEQ ID NO: 64;

(bm) a polypeptide having at least 80% sequence identity to SEQ ID NO: 65;

(bn) a polypeptide having at least 80% sequence identity to SEQ ID NO: 66;

(bo) a polypeptide having at least 80% sequence identity to SEQ ID NO: 67;

(bp) a polypeptide having at least 80% sequence identity to SEQ ID NO: 68;

(bq) a polypeptide having at least 80% sequence identity to SEQ ID NO: 69;

(br) a polypeptide having at least 80% sequence identity to SEQ ID NO: 70;

(bs) a polypeptide having at least 80% sequence identity to SEQ ID NO: 71;

(bt) a variant of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48, SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 64, SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 69, SEQ ID NO: 70 or SEQ ID NO: 71 comprising one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 positions;

(bu) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l), (m), (n), (o), (p), (q), (r), (s), (t), (u), (v), (w), (x), (y), (z), (aa), (ab), (ac), (ad), (ae), (af), (ag), (ah), (ai), (aj), (ak), (al), (am), (an), (ao), (ap), (aq), (ar), (as), (at), (au), (av), (aw), (ax), (ay), (az), (ba), (bb), (bc), (bd), (be), (bf), (bg), (bh), (bi), (bj), (bk), (bl), (bm), (bn), (bo), (bp), (bq), (br), (bs) or (bt) and a N-terminal and/or C-terminal extension of between 1 and 10 amino acids; and

(bv) a fragment of a polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l), (m), (n), (o), (p), (q), (r), (s), (t), (u), (v), (w), (x), (y), (z), (aa), (ab), (ac), (ad), (ae), (af), (ag), (ah), (ai), (aj), (ak), (al), (am), (an), (ao), (ap), (aq), (ar), (as), (at), (au), (av), (aw), (ax), (ay), (az), (ba), (bb), (bc), (bd), (be), (bf), (bg), (bh), (bi), (bj), (bk), (bl), (bm), (bn), (bo), (bp), (bq), (br), (bs) or (bt) having muramidase activity and having at least 90% of the length of the mature polypeptide.

6. The animal feed or animal feed additive of claim 1 , wherein the probiotic comprises one or more Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus pumilus, Bacillus polymyxa, Bacillus megaterium, Bacillus coagulans, Bacillus circulans, Lactobacillus farciminus, Lactobacillus rhamnosus, Clostridium butyricum, Lactobacillus reuteri , and/or Lactobacillus salivarius ssp. salivarius.

7. The animal feed or animal feed additive of claim 1 , wherein the probiotic is selected from the group consisting of a combination of one or more Lactobacillus and one or more Saccharomyces , one or more Lactobacillus and one or more Streptococcus , one or more Lactobacillus and one or more Bacillus , one or more Lactobacillus and one or more Bifidobacterium , one or more Saccharomyces and one or more Bacillus , one or more Saccharomyces and one or more Bacillus , one or more Streptococcus and one or more Bacillus , one or more Bacillus and one or more Bifidobacterium.

8. The animal feed or animal feed additive of claim 1 , further comprising a protein source selected from the group consisting of soybean, wild soybean, beans, lupin, tepary bean, scarlet runner bean, slimjim bean, lima bean, French bean, Broad bean (fava bean), chickpea, lentil, peanut, Spanish peanut, canola, sunflower seed, cotton seed, rapeseed (oilseed rape) or pea or in a processed form such as soybean meal, full fat soy bean meal, soy protein concentrate (SPC), fermented soybean meal (FSBM), sunflower meal, cotton seed meal, rapeseed meal, fish meal, bone meal, feather meal, whey or any combination thereof.

9. The animal feed or animal feed additive of claim 1 , further comprising an energy source selected from the group consisting of maize, corn, sorghum, barley, wheat, oats, rice, triticale, rye, beet, sugar beet, spinach, potato, cassava, quinoa , cabbage, switchgrass, millet, pearl millet, foxtail millet or in a processed form such as milled corn, milled maize, potato starch, cassava starch, milled sorghum, milled switchgrass, milled millet, milled foxtail millet, milled pearl millet, or any combination thereof.

10. The animal feed or animal feed additive of claim 1 , further comprising one or more components selected from the list consisting of:

one or more additional enzymes;

one or more microbes;

one or more vitamins;

one or more minerals;

one or more amino acids; and

one or more other feed ingredients.

11. The animal feed or animal feed additive of claim 1 , wherein the polypeptide having GH24 muramidase activity and/or GH25 muramidase activity is formulated as a granule.

12. A method of improving European production efficiency factor (EPEF), body weight gain (BWG) and/or feed conversion ratio (FCR) of a mono-gastric animal comprising feeding the animal feed or animal feed additive of claim 1 to said mono-gastric animal.

13. An animal feed or animal feed additive comprising one or more fungal polypeptides having GH24 muramidase activity and/or GH25 muramidase activity and one or more probiotics selected from Lactobacillus, Bacillus, Clostridium , and combinations thereof.

14. The animal feed or animal feed additive of claim 13 , wherein the probiotic comprises one or more Bacillus.

15. The animal feed or animal feed additive of claim 13 , wherein the probiotic comprises one or more Lactobacillus.

16. The animal feed or animal feed additive of claim 13 , wherein the polypeptide having GH24 muramidase activity and/or GH25 muramidase activity is selected from the group consisting of: