Increasing plant growth and yield by using a DUF2996 domain-containing protein
Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding DUF2996 domain-containing protein s, polypeptides encompassing DUF2996 domain-containing protein s, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
1. A method for increasing crop yield comprising transforming a plant with at least one coding sequence encoding a DUF2996 domain-containing protein wherein said coding sequence encoding a DUF2996 domain-containing protein shares at least 95% identity with SEQ ID NO:1, or encodes a protein having an amino acid sequence that shares at least 95% identity with the amino acid sequence of SEQ ID NO: 2, and wherein expression of said coding sequence increases yield in said plant;
wherein said DUF2996 domain-containing protein possesses the biological activity of catalyzing the conversion of quinone and NAD(P)H to quinol and NAD(P) + ;
wherein said coding sequence encoding a DUF2996 domain-containing protein is expressed from a bundle sheath cell-preferred promoter comprising SEQ ID NO: 3.
2. A plant having stably incorporated into its genome a promoter that drives expression in a plant operably linked to a coding sequence encoding a DUF2996 domain-containing protein wherein said coding sequence encoding a DUF2996 domain-containing protein shares at least 95% identity with SEQ ID NO:1, or encodes a protein having an amino acid sequence that shares at least 95% identity with the amino acid sequence of SEQ ID NO:2 wherein said promoter is heterologous to said coding sequence encoding a DUF2996 domain-containing protein;
wherein said DUF2996 domain-containing protein possesses the biological activity of catalyzing the conversion of quinone and NAD(P)H to quinol and NAD(P) + ;
wherein said promoter that drives expression in a plant is a bundle sheath cell-preferred promoter comprising SEQ ID NO:3.
3. The plant of claim 2 wherein said plant is a monocot.
4. The plant of claim 2 wherein said plant is a dicot.
5. A DNA construct comprising, in operable linkage,
a. a promoter that is functional in a plant cell and comprises SEQ ID NO: 3, and
b. a nucleic acid sequence encoding a DUF2996 domain-containing protein, wherein said nucleic acid sequence encoding a DUF2996 domain-containing protein comprises a sequence that shares at least 95% identity with SEQ ID NO:1, or encodes a protein having an amino acid sequence that shares at least 95% identity with the amino acid sequence of SEQ ID NO:2;
wherein said DUF2996 domain-containing protein possesses the biological activity of catalyzing the conversion of quinone and NAD(P)H to quinol and NAD(P) + ;
wherein said promoter is heterologous to said nucleic acid sequence encoding the DUF2996 domain-containing protein.
6. The method of claim 1 wherein said coding sequence encoding a DUF2996 domain-containing protein comprises SEQ ID NO:1, or encodes a protein having the amino acid sequence of SEQ ID NO:2.
7. The plant of claim 2 wherein said coding sequence encoding a DUF2996 domain-containing protein comprises SEQ ID NO:1, or encodes a protein having the amino acid sequence of SEQ ID NO:2.
8. The DNA construct of claim 5 wherein said nucleic acid sequence encoding a DUF2996 domain-containing protein comprises SEQ ID NO:1, or encodes a protein having the amino acid sequence of SEQ ID NO:2.
9. The method of claim 1 , wherein the crop yield increase in said plant comprises increase in above-ground biomass, and/or increase in harvestable biomass, and/or increase in seed yield.
10. The plant of claim 2 , wherein the crop yield increase in said plant comprises increase in above-ground biomass, and/or increase in harvestable biomass, and/or increase in seed yield.