AUTOMATED AND DYNAMICALLY ADJUSTABLE GAS MIXER FOR BIOREACTOR SYSTEM
A gas mixer system includes a first line carrying a first gas component, and a first flow rate controller adapted to receive data relating to a measure of a first parameter and adjust a first flow rate of the first gas component based on the measure of the first parameter. The gas mixer system also includes a second line carrying a second gas component, and a second flow rate controller adapted to receive data relating to a measure of a second parameter and adjust a second flow rate of the second gas component based on the measure of the second parameter. The gas mixer system further includes a vessel connected to the first and second flow rate controllers, wherein the first gas component and the second gas component are mixed in the vessel to produce a gas mixture, and at least one outlet port. For example, the gas mixer may be connected to and provide a gas mixture to a bioreactor.
1 . A bioreactor system including:
a bioreactor comprising:
a bioreactor environment comprising a volume adapted to hold one or more gases;
a cell culture disposed in the bioreactor environment; and
a sensor adapted to generate a measure of a parameter of the bioreactor environment; and
a gas mixer comprising:
a first line carrying a first gas component;
a first flow rate controller adapted to adjust a first flow rate of the first gas component based on the measure of the parameter;
a second line carrying a second gas component;
a second flow rate controller adapted to adjust a second flow rate of the second gas component;
a vessel connected to the first and second flow rate controllers, wherein the first gas component and the second gas component are mixed in the vessel to produce a gas mixture; and
at least one outlet port through which the gas mixture is provided to the bioreactor environment.
2 . The bioreactor system of claim 1 , wherein:
the first gas component includes one of: ambient air, oxygen, carbon dioxide, and nitrogen; and
the second gas component includes one of: ambient air, oxygen, carbon dioxide, and nitrogen.
3 . The bioreactor system of claim 1 , wherein the parameter includes one of: a pressure of the bioreactor environment, a proportion of oxygen in the bioreactor environment, a proportion of carbon dioxide in the bioreactor environment, a proportion of nitrogen in the bioreactor environment, a measure of a growth rate of the cell culture, a pH level of the cell culture, and a temperature in the bioreactor environment.
4 . The bioreactor system of claim 3 , wherein the sensor comprises one of: a pressure sensor, an oxygen sensor, a carbon dioxide sensor, a nitrogen sensor, a growth rate sensor, a pH sensor, and a temperature sensor.
5 . The bioreactor system of claim 1 , wherein:
the first gas component is carbon dioxide;
the parameter is a pH level of the cell culture; and
the first flow rate controller is adapted to adjust a first flow rate of the carbon dioxide based on the pH level.
6 . The bioreactor system of claim 1 , wherein:
the first gas component is oxygen;
the parameter is a measure of a growth rate of the cell culture; and
the first flow rate controller is adapted to adjust a first flow rate of the oxygen based on the measure of the growth rate.
7 . The bioreactor system of claim 1 , further comprising:
a pressure regulator disposed between the vessel and the at least one outlet port.
8 . A gas mixer system comprising:
a first line carrying a first gas component;
a first flow rate controller adapted to:
receive data relating to a measure of a first parameter; and
adjust a first flow rate of the first gas component based on the measure of the first parameter;
a second line carrying a second gas component;
a second flow rate controller adapted to:
receive data relating to a measure of a second parameter; and
adjust a second flow rate of the second gas component based on the measure of the second parameter;
a vessel connected to the first and second flow rate controllers, wherein the first gas component and the second gas component are mixed in the vessel to produce a gas mixture; and
at least one outlet port through which the gas mixture flows.
9 . The gas mixer system of claim 8 , wherein:
the first gas component includes one of: air, oxygen, carbon dioxide, and nitrogen; and
the second gas component includes one of: air, oxygen, carbon dioxide, and nitrogen.
10 . The gas mixer system of claim 8 , wherein:
the first parameter includes one of: a pressure of the bioreactor environment, a proportion of oxygen in the bioreactor environment, a proportion of carbon dioxide in the bioreactor environment, a proportion of nitrogen in the bioreactor environment, a measure of a growth rate of the cell culture, a pH level of the cell culture, and a temperature in the bioreactor environment; and
the second parameter includes one of: a pressure of the bioreactor environment, a proportion of oxygen in the bioreactor environment, a proportion of carbon dioxide in the bioreactor environment, a proportion of nitrogen in the bioreactor environment, a measure of a growth rate of the cell culture, a pH level of the cell culture, and a temperature in the bioreactor environment.