Treatment of uterine fibroids using purified collagenase
Disclosed herein are compositions and methods for treating uterine fibroids in vivo, and methods of reducing symptoms associated with uterine fibroids, including pain, bleeding and infertility. The disclosed compositions comprise collagenase in an amount effective to cause shrinkage and/or reduce stiffness of uterine fibroids.
1. A method for reducing the size of a uterine fibroid in a patient, the method comprising:
administering into the uterine fibroid a composition comprising Clostridium histolyticum collagenase I and collagenase II in a 1:1 mass ratio to thereby reduce the size of the uterine fibroid, wherein there is an at least two fold increase in LC3B expression within the uterine fibroid as measured at 60 days following the administration.
2. The method of claim 1 , wherein said composition is delivered through a delivery channel into said fibroid, wherein the delivery channel is in a needle, syringe, cannula, catheter or jet injector.
3. The method of claim 1 , wherein about 0.005 mg to about 10 mg collagenase is administered per cm 3 of tissue to be treated.
4. The method of claim 1 , wherein about 0.05 mg to about 1 mg collagenase is administered per cm 3 of tissue to be treated.
5. The method of claim 1 , wherein about 0.25 mg to about 1 mg collagenase is administered per cm 3 of tissue to be treated.
6. The method of claim 1 , wherein the method results in an at least three fold reduction in expression of proliferating cell nuclear antigen (PCNA) as measured at 60 days following the administration.
7. A method for reducing pain associated with a uterine fibroid in a patient, the method comprising:
administering into the uterine fibroid in the patient a composition comprising Clostridium histolyticum collagenase I and collagenase II in a 1:1 mass ratio to thereby reduce the pain associated with the uterine fibroid, wherein the patient has a reduction of at least 1 point on the McGill Pain Scale as measured at 60 days following the administration.
8. The method of claim 7 , wherein said composition is delivered through a delivery channel into said fibroid, wherein the delivery channel is in a needle, syringe, cannula, catheter or jet injector.
9. The method of claim 7 , wherein about 0.005 mg to about 10 mg collagenase is administered per cm 3 of tissue to be treated.
10. The method of claim 7 , wherein about 0.05 mg to about 1 mg collagenase is administered per cm 3 of tissue to be treated.
11. The method of claim 7 , wherein about 0.25 mg to about 1 mg collagenase is administered per cm 3 of tissue to be treated.
12. The method of claim 7 , wherein at 60 days following the administering the patient has a reduction of about 15 points on the McGill Pain Scale.