Temporal sensing and related methods
Embodiments described herein generally relate to: sensing and/or authentication using luminescence imaging; diagnostic assays, systems, and related methods; temporal thermal sensing and related methods; and/or to emissive species, such as those excitable by white light, and related systems and methods.
1. A method for identifying a change in an emissive species over a period of time, comprising:
exciting the species such that it produces a detectable non-steady-state emission during an emission time period of the emissive species, wherein the emission time period is at least 10 nanoseconds;
obtaining, using an image sensor, data associated with the detectable non-steady state emission, wherein the image sensor is configured to sequentially read a first row or first column of pixels, and a second row or second column of pixels, from an array of pixels of the image sensor;
creating, based on at least a portion of the data obtained using the image sensor, a single image, wherein a first set of data used to create a first portion of the single image corresponds to a first portion of the emission time period captured in the first row or first column of pixels, and wherein a second set of data used to create a second portion of the single image corresponds to a second portion of the emission time period captured in the second row or second column of pixels; and
determining, based upon a difference between the first portion and the second portion of the single image, the change in the emissive species.
2. A method for identifying a change in an emissive species over a period of time, comprising:
causing the species to emit non-steady-state electromagnetic radiation during an emission time period of the emissive species;
obtaining, using an image sensor, a single image of at least a portion of the electromagnetic radiation emitted by the emissive species, wherein the image sensor is configured to sequentially read a first row or first column of pixels, and a second row or second column of pixels, from an array of pixels of the image sensor;
identifying information from the first row or first column of pixels corresponding to emission of electromagnetic radiation by the emissive species at least at a first point in time;
identifying information from the second row or second column of pixels corresponding to emission of electromagnetic radiation by the emissive species at least at a second point in time; and
determining, from at least the information from the first row or first column of pixels and the information from the second row or second column of pixels, the change in the emissive species.
3. A method as in any preceding claim, comprising:
identifying information from more than two image portions of the single image corresponding to emission of electromagnetic radiation by the emissive species at more than two points in time, and/or obtaining a plurality of images, each image being of at least a portion of the electromagnetic radiation emitted by the emissive species, and for each image identifying information from a first image portion corresponding to emission of electromagnetic radiation by the emissive species at least at a first point in time, and identifying information from a second image portion corresponding to emission of electromagnetic radiation by the emissive species at least at a second point in time; and from information identified from the more than two image portions, and/or from information from the plurality of images, determining a change in the emissive species.
4. A method as in claim 1 , wherein the emissive species produces a detectable steady-state emission.
5. A method as in claim 1 , comprising a second emissive species, different than the emissive species, wherein the second emissive species produces a detectable steady-state emission under a set of conditions.
6. A method as in claim 1 , wherein the image sensor is configured to detect the detectable steady-state emission.
7. A method as in claim 1 , wherein the emission time period is at least 10 nanoseconds.
8. A method as in claim 1 , wherein exciting the species comprises exposing the species to electromagnetic radiation.
9. A method as in claim 1 , wherein the exciting electromagnetic radiation is provided as a single pulse, a periodic pulse, a sequence of pulses, a pulse of continuously varying intensity, or any combination thereof.
10. A method as in claim 1 , wherein the electromagnetic radiation is modulated by an electrical signal, shutter, refractory material, optical modulator, moving minor, mechanical device, or light valve.
11. A method as in claim 1 , wherein the electromagnetic radiation comprises visible light.
12. A method as in claim 1 , wherein the electromagnetic radiation comprises substantially white light.
13. A method as in claim 1 , wherein the electromagnetic radiation comprises discrete wavelength ranges.
14. A method as in claim 1 , wherein exciting the species comprises exposing the species to pulsed and/or modulated light from an LED, an OLED, a fluorescent light, and/or an incandescent bulb.
15. A method as in claim 1 , wherein exciting the species comprises exposing the species to a flash lamp.
16. A method as in claim 1 , wherein exciting the species comprises applying a voltage, ionizing radiation, a physical force, or chemical reaction.
17. A method as in claim 1 , wherein the species is associated with a packaging component.
18. A method as in claim 1 , wherein the species undergoes a chemical and/or biological reaction upon excitation.
19. A method as in claim 1 , wherein exposure to an analyte causes a change in one or more of an intensity of the emitted light, a polarization of the emitted light, a spatial profile of the emitted light or a change in the emission lifetime of the emissive species.
20. A method as in claim 1 , further comprising a second step of activating an article.