Propionibacterial cell for organic acid production
Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.
1. A Propionibacterium acidipropionici ( P. acidipropionici ) cell comprising one or more of the loss of function mutations in genes encoding DUF1116 domain-containing protein (A5Q49_R502075; SEQ ID NO:26) and M18 family aminopeptidase (A5Q49_RS15965; SEQ ID NO:24) identified in Table 3.
2. The P. acidipropionici cell of claim 1 further comprising one or more additional mutations identified in Table 3.
3. The P. acidipropionici cell of claim 1 , wherein the cell comprises a loss of function mutation in each of the genes encoding the proteins identified in Table 3.
4. A P. acidipropionici cell comprising at least one loss of function mutation in gene(s) encoding protein(s) selected from the group consisting of O-antigen ligase domain-containing protein (ASQ49_RS02520; SEQ ID NO:9), amino acid permease (ASQ49_RS13125; SEQ ID NO:17), DUF1116 domain-containing protein (A5Q49_R502075; SEQ ID NO:26) and M18 family aminopeptidase (A5Q49_RS15965; SEQ ID NO:24), and adenine glycosylase (ASQ49_RS07985; SEQ ID NO:19).
5. The P. acidipropionici cell of claim 4 comprising a loss of function mutation in the gene encoding O-antigen ligase domain-containing protein (ASQ49_RS02520; SEQ ID NO:9).