Compositions on plasma-treated surfaces
The disclosure provides solid compositions such as lyophilisates adhered to surfaces such as plasma-treated surfaces and related methods, uses, kits, intermediates, starting materials, and downstream products.
1. A solid composition adhered to a plasma-treated surface, wherein the plasma-treated surface is an interior surface of a vessel, wherein the solid composition has a mass from about 600 micrograms to about 20 grams, wherein the solid composition is in the form of a lyophilized pellet adhered to the plasma-treated interior surface, wherein the solid composition is macroscopic in three orthogonal dimensions, such that the solid composition is not a coating and has a length, a width, and a height of greater than 1 mm, and wherein the solid composition is sufficiently adhered to remain substantially attached to the plasma-treated surface following a package performance test in which the plasma-treated surface containing the solid composition is dropped from a height of about 20 mm to about 1000 mm onto a substrate.
2. The solid composition of claim 1 , wherein the solid composition comprises a bulking agent and at least one of an enzyme and an oligonucleotide.
3. The solid composition of claim 1 , wherein the plasma-treated surface comprises plastic.
4. The solid composition of claim 3 , wherein the plastic comprises at least one of a polyethylene, polyethylene terephtalate, polypropylene, polymethacrylate, polyvinyl chloride, polystyrene, polyolefin, polycarbonate, polyurethane, starch-derived plastic, or cyclic olefin copolymer.
5. The solid composition of claim 2 , wherein the bulking agent comprises a saccharide, glycine, or hydroxyethyl starch.
6. The solid composition of claim 2 , wherein the bulking agent comprises a saccharide comprising at least one of sucrose, mannitol, raffinose, or trehalose.
7. The solid composition of claim 1 , wherein the vessel comprises a tube having a volume ranging from about 40 ul to about 60 ml.
8. The solid composition of claim 1 , wherein the vessel is a multiwell plate comprising a plurality of wells.
9. The solid composition of claim 1 , wherein the solid composition has a mass ranging from about 5 mg to about 20 g, about 200 mg to about 20 g, about 1 g to about 20 g, about 5 g to about 20 g, about 5 mg to about 1 g, about 5 mg to about 500 mg, about 5 mg to about 200 mg, about 5 mg to about 100 mg, about 5 mg to about 50 mg, about 5 mg to about 20 mg, about 5 mg to about 10 mg, about 10 mg to about 200 mg, about 20 mg to about 200 mg, about 50 mg to about 200 mg, or about 100 mg to about 200 mg.
10. The solid composition of claim 1 , wherein the solid composition comprises one or more enzymes.
11. The solid composition of claim 10 , wherein the one or more enzymes include at least one of a DNA polymerase, an RNA polymerase, a ligase, a kinase, a phosphatase, a protease, an exonuclease, and an endonuclease.
12. The solid composition of claim 1 , wherein the solid composition comprises a buffer.
13. The solid composition of claim 1 , wherein the solid composition comprises a salt.
14. The solid composition of claim 1 , wherein the solid composition is free of inorganic salt or has an inorganic salt concentration of 5 mM or less.
15. The solid composition of claim 1 , wherein the solid composition comprises at least one oligonucleotide useful for performing a molecular assay.
16. The solid composition of claim 1 , wherein the solid composition comprises one or more nucleoside triphosphates.
17. The solid composition of claim 1 , wherein the solid composition contains a single unit dose of amplification or detection reagents.
18. The solid composition of claim 1 , wherein the solid composition contains a pharmaceutically active agent.
19. The solid composition of claim 1 , wherein the plasma-treated surface is a surface comprising a polyolefin and has a contact angle of 5° to 50°.
20. The solid composition of claim 1 , wherein the plasma-treated surface is a surface comprising a polyolefin and has a surface energy from 33 to 55 dynes/cm.
21. The solid composition of claim 1 , wherein the plasma-treated surface is a surface comprising a polyethylene or polypropylene and has a surface energy from 35 to 55 dynes/cm.
22. The solid composition of claim 1 , wherein the plasma-treated surface is a surface treated with a cold cathode discharge, hollow cathode discharge, DC-induced discharge, radio frequency (RF)-induced discharge, corona discharge, glow discharge, or charged particle beam.
23. The solid composition of claim 1 , wherein the plasma-treated surface is a surface treated with a corona discharge at a watt density ranging from about 25 watt/min/m2 to about 2000 watt/min/m2, about 50 watt/min/m2 to about 1500 watt/min/m2, about 100 to about 1200 watt/min/m2, about 200 to about 1000 watt/min/m2, about 100 to about 600 watt/min/m2, or about 200 watt/min/m2 to about 600 watt/min/m2 or, wherein the surface was treated with a corona discharge at a watt density ranging from about 25 watt/min/m2 to about 2000 watt/min/m2, about 50 watt/min/m2 to about 1500 watt/min/m2, about 100 to about 1200 watt/min/m2, about 200 to about 1000 watt/min/m2, about 100 to about 600 watt/min/m2, or about 200 watt/min/m2 to about 600 watt/min/m2.