Biomedical patches with aligned fibers
A multi-laminar electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate is provided. The scaffold includes a first layer formed by a first plurality of electrospun polymeric fibers, and a second layer formed by a second plurality of electrospun polymeric fibers. The second layer is combined with the first layer. A first portion of the scaffold includes a higher density of fibers than a second portion of the scaffold, and the first portion has a higher tensile strength than the second portion. The scaffold is configured to degrade via hydrolysis after at least one of a predetermined time or an environmental condition. The scaffold is configured to be applied to the tissue substrate containing the defect, and is sufficiently flexible to facilitate application of the scaffold to uneven surfaces of the tissue substrate, and to enable movement of the scaffold by the tissue substrate.
1. A biomedical patch device for repairing a wound in tissue, the biomedical patch device comprising:
an array of a plurality of depressions located on a surface portion of the biomedical patch device, the array of the plurality of depressions arranged in an ordered configuration on the surface portion of the biomedical patch device, the array of the plurality of depressions configured for promoting adherence of cells to the surface portion of the biomedical patch device; and
a polymeric scaffold located in a subsurface portion of the biomedical patch device, the polymeric scaffold formed by a plurality of nonwoven electrospun fibers, the plurality of nonwoven electrospun fibers comprising fibers extending in a plurality of axes in three dimensions to facilitate cellular migration and cellular infiltration along the plurality of axes, the plurality of axes comprising a radial axis extending from a point in the biomedical patch device;
the plurality of nonwoven electrospun fibers connecting the array of the plurality of depressions and the polymeric scaffold;
the plurality of nonwoven electrospun fibers comprising a first set of biodegradable fibers generated by electrospinning a first polymer composition and a second set of biodegradable fibers generated by electrospinning a second polymer composition, wherein at least some of the first set of biodegradable fibers are overlaid on at least some of the second set of biodegradable fibers;
the array of depressions and the polymeric scaffold comprising sufficient pliability to apply the biomedical patch device to uneven surfaces of tissue.
2. The biomedical patch device of claim 1 , wherein the plurality of nonwoven electrospun fibers further comprises one or more non-radially aligned fibers.
3. The biomedical patch device of claim 1 , wherein at least some of the first set of biodegradable fibers and at least some of the second set of biodegradable fibers are deposited simultaneously.
4. The biomedical patch device of claim 1 , wherein a first portion of the biomedical patch device of a particular size comprises a higher number of fibers than a second portion of the biomedical patch device of the particular size.
5. The biomedical patch device of claim 4 , wherein the array of the plurality of depressions comprises the plurality of nonwoven electrospun fibers.
6. The biomedical patch device of claim 5 , wherein the plurality of nonwoven electrospun fibers is deposited directly on a collector without a mask.
7. The biomedical patch device of claim 6 , wherein the collector comprises a plurality of electrodes arranged in the ordered configuration.
8. The biomedical patch device of claim 6 , wherein a depth of the plurality of depressions comprises about 200 micrometers.
9. The biomedical patch device of claim 6 , wherein a depth of the plurality of depressions comprises about 400 micrometers.
10. The biomedical patch device of claim 6 , wherein the first polymer composition comprises glycolic acid, and wherein the second polymer composition comprises caprolactone.
11. The biomedical patch device of claim 6 , wherein the polymeric scaffold further comprises a plurality of topographical features formed by the plurality of nonwoven electrospun fibers.
12. The biomedical patch device of claim 6 , wherein the polymeric scaffold further comprises a surface area to volume ratio substantially similar to an extracellular matrix.
13. The biomedical patch device of claim 1 , wherein the radial axis is substantially parallel to the surface portion of the biomedical patch device.
14. The biomedical patch device of claim 6 , wherein the array of depressions and the polymeric scaffold are suturable.
15. The biomedical patch device of claim 6 , wherein the first polymer comprises glycolic acid.
16. The biomedical patch device of claim 1 , wherein the polymeric scaffold further comprises a plurality of radially aligned fiber sections and a plurality of randomly oriented fiber sections.
17. The biomedical patch device of claim 16 , wherein one or more of the plurality of radially aligned fiber sections are overlaid on one or more of the plurality of randomly oriented fiber sections.
18. The biomedical patch device of claim 16 , wherein one or more of the plurality of randomly oriented fiber sections are overlaid on one or more of the plurality of radially aligned fiber sections.
19. The biomedical patch device of claim 16 , wherein one or more of the plurality of randomly oriented fiber sections extends into one or more of the plurality of radially aligned fiber sections.
20. The biomedical patch device of claim 16 , wherein the plurality of radially aligned fiber sections extend radially outwardly from a point within the polymeric scaffold.
21. The biomedical patch device of claim 16 , wherein one or more of the plurality of radially aligned fiber sections and one or more of the plurality of randomly oriented fiber sections overlap.
22. The biomedical patch device of claim 1 , wherein the biomedical patch device is watertight for use as a dura substitute, wherein the tissue comprises dura mater.
23. The biomedical patch device of claim 22 , wherein the biomedical patch device further comprises a layer of gel.
24. The biomedical patch device of claim 1 , wherein the polymeric scaffold further comprises a plurality of areas comprising radially aligned fiber sections extending radially outwardly from a point within the polymeric scaffold.
25. The biomedical patch device of claim 1 , wherein the tissue comprises skin.
26. The biomedical patch device of claim 1 , wherein the biomedical patch device is configured to be applied to one or more of a bone fracture, a defect in skin, trachea, gastrointestinal tract, or cardiac defect.
27. The biomedical patch device of claim 1 , wherein the plurality of nonwoven electrospun fibers comprises one or more fibers comprising a substantially straight fiber section and a randomly oriented fiber section.