Ultra-sensitive platform for nucleic acid detection using a novel method, scanning digital polymerase chain reaction (PCR)
A method for analyzing a target nucleic acid includes diluting nucleic acid targets and filling pico to femto-liter sized wells such that they contain a single target nucleic acid and one or more amplification reagents, amplifying the target in the individual wells, distinguishing wells containing amplicon from the target and amplicon from a variant of the target generated by polymerase error by using two differently labeled-hybridization probes, one hybridizing to the target and one hybridizing to a specific variant of the target; and analyzing target amplicons.
1. A system for detecting a target nucleic acid, comprising:
a cassette, the cassette comprising a sample input configured to receive an emulsion containing at least one target molecule and at least one non-target molecule, the cassette further comprising at least one intermediate layer, the cassette further comprising at least one capping layer, the at least one capping layer and intermediate layer defining at least one chamber, the at least one intermediate layer having a droplet orifice formed therethrough allowing fluid communication between the sample input and a first chamber of the at least one chamber, the at least one intermediate layer defining at least one egress orifice allowing the emulsion to exit the at least one chamber; and
a flow cytometer having an interior configured to receive at least a portion of the cassette, the flow cytometer configured to identify the at least one target molecule.
2. The system of claim 1 , wherein the droplet orifice is sized to pass the emulsion molecules from the sample input to the chamber in single file.
3. The system of claim 1 , wherein the flow cytometer identifies the at least one target molecule by scanning molecules of the emulsion with a laser.
4. The system of claim 1 , wherein the egress orifice is part of a Coulter-style particle interrogation structure that interrogates each molecule of the emulsion passing through the egress orifice.
5. The system of claim 1 , further comprising a pressure generator configured to create emulsion droplets by drawing the emulsion molecules from the sample input through the egress orifice.
6. The system of claim 1 , wherein the cassette further comprises a piezoelectric transducer configured to cause only the identified at least one target molecule to be drawn through the droplet orifice.