Flow cell carrier and methods of use
The present disclosure relates to a flow cell carrier. The flow cell carrier may include a flow cell and a frame. The frame may include a pocket and a handle. The pocket may have at least one spring feature and at least one banking feature. The frame may be configured to retain the flow cell within the pocket such that a maximal surface area of the flow cell is exposed to an optical lens. Related methods and kits are also disclosed.
1. A flow cell carrier comprising:
a flow cell; and
a frame, the frame comprising:
a handle;
wherein the frame is configured to retain the flow cell and wherein the frame further comprises at least one ferromagnetic pin.
2. The flow cell carrier of claim 1 , wherein the frame further comprises a pocket, the pocket comprising at least one biasing feature.
3. The flow cell carrier of claim 2 , wherein the frame is configured to retain the flow cell within the pocket.
4. The flow cell carrier of claim 3 , wherein the frame is configured to retain the flow cell within the pocket such that a maximal surface area of the flow cell is available to be exposed to an optical lens of a sequencing device.
5. The flow cell carrier of claim 1 , wherein the retaining of the flow cell within the pocket further comprises constraining a first, a second, a third, a fourth, a fifth, and a sixth degree of freedom of the flow cell.
6. The flow cell carrier of claim 1 , wherein the frame is an injection molded frame.
7. The flow cell carrier of claim 1 , wherein the handle is a raised handle.
8. The flow cell carrier of claim 1 , wherein the frame is further configured to provide a gap between a work surface and the flow cell.
9. The flow cell carrier of claim 2 , wherein the at least one biasing feature is a spring finger.
10. The flow cell carrier of claim 2 , wherein the at least one biasing feature is a tab.
11. The flow cell carrier of claim 1 , further comprising a microchip.
12. The flow cell carrier of claim 11 , wherein the microchip is an electronically erasable programmable read only memory (EEPROM) chip.
13. A kit for sequencing a nucleic acid comprising:
the flow cell carrier of claim 1 , wherein the flow cell is configured to serve as a reaction vessel in a nucleic acid sequencing device;
a sequencing solution comprising one or more reagents and one or more enzymes for DNA sequencing.
14. The kit of claim 13 , further comprising an aqueous solution comprising one or more reagents and one or more enzymes for DNA amplification.
15. The flow cell carrier of claim 1 , wherein the flow cell comprises a plurality of inlet ports sized to receive a flow of one or more reagents and one or more enzymes for DNA amplification and a plurality of outlet ports that pass the flow of reagents out of the flow cell.
16. The flow cell carrier of claim 1 , wherein the flow cell comprises a plurality of inlet ports sized to receive a flow of one or more reagents, one or more washes, and one or more enzymes for DNA sequencing and a plurality of outlet ports that pass the flow of reagents out of the flow cell.
17. The flow cell carrier of claim 1 , wherein the flow cell carrier is configured to removably engage within a nucleic acid sequencing device.
18. The flow cell carrier of claim 1 , wherein the ferromagnetic pin of the frame comprises an alloy containing iron, cobalt, or nickel and a metalloid.