Detecting colorectal neoplasm
Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting premalignant and malignant neoplasms such as colorectal cancer.
1. A method comprising:
extracting genomic DNA from a biological sample of a human individual suspected of having or having colorectal cancer or a large colorectal adenoma;
treating the extracted genomic DNA with bisulfite;
amplifying the bisulfite-treated genomic DNA using primers specific for SFMBT2 and CHST2; and
measuring a methylation level for SFMBT2 and CHST2 using methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, or bisulfite genomic sequencing PCR.
2. The method of claim 1 , wherein the biological sample is a stool sample, a tissue sample, a colorectal cyst sample, a colorectal tumor sample, a blood sample, or a urine sample.
3. The method of claim 1 , wherein the sample comprises colorectal tissue.
4. The method of claim 1 , wherein:
the primers specific for SFMBT2 are capable of binding an amplicon bound by SEQ ID NOs: 10 and 11, SEQ ID NOs: 16 and 17, SEQ ID NOs: 30 and 31, SEQ ID NOs: 60 and 62, SEQ ID NOs: 63 and 65, or SEQ ID NOs: 90 and 92; and
the primers specific for CHST2 are capable of binding an amplicon bound by SEQ ID NOs: 20 and 21, SEQ ID NOs: 28 and 29, SEQ ID NOs: 57 and 59; or SEQ ID NOs: 69 and 71.
5. The method of claim 1 , wherein the method further comprises amplifying the bisulfite-treated genomic DNA using primers specific for NDRG4, and measuring a methylation level for NDRG4.